Clonogenicity of adipose stem cells. (A) Phase contrast image of a representative single-cell derived clone. (B) Quantitative evaluation of the clonogenicity of stem cells isolated from lipoaspirates (L) and from lipoaspirates enriched with stem cells (L + SC). Data expressed as mean ± standard deviation. *P <0.05 vs. L. (C, D, E, F) Clones are characterized by a primitive phenotype: highly expressing Oct-4 (red fluorescence, C), Nanog (green fluorescence, D), Sox-2 (yellow fluorescence, E), c-Kit (red fluorescence, F), and the intermediate filaments nestin (green fluorescence, G) and vimentin (red fluorescence, H). Cytori, Cytori Celution System (Cytori Ltd., Deeside, UK) ; Fastem, Fastem Corios (CORIOS Soc. Coop, San Giuliano Milanese (MI), Italy); Lipokit, Lipokit Medikhan System (Medikan International Inc, Pusan, Korea) .