Figure 4

Multipotency of adipose stem cells. Stem cells isolated from lipoaspirates (L) (A, D, F, I, L) and from lipoaspirates enriched with stem cells (L + SC) (B, E, G, J, M) were evaluated for their ability to differentiate into adipocyte-like cells staining positive for Oil-red O (red vacuoles, A, B) and peroxisome proliferator-activated receptor gamma (magenta, D, E), endothelial-like cells positive for CD31 (green fluorescence, F, G), and muscle cells positive for smooth muscle actin (SMA; red fluorescence, I, J) and alpha sarcometic actin (ASA; red fluorescence, L, M). Nuclei are depicted by the blue staining of either hematoxylin or 4′,6-diamidino-2-phenylindole. (C, H, K, N) Results expressed as mean ± standard deviation. *P <0.05 vs. L. Cytori, Cytori Celution System (Cytori Ltd., Deeside, UK) [25]; Fastem, Fastem Corios (CORIOS Soc. Coop, San Giuliano Milanese (MI), Italy); Lipokit, Lipokit Medikhan System (Medikan International Inc, Pusan, Korea) [26].