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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

Figure 4

Percentage of CD4 + and CD8 + T cells expressing interleukin-17 (IL-17). The left upper panels show the percentage (mean ± standard deviation) of CD4+ and CD8+ T cells producing IL-17 (within total CD4+ T cells and total CD8+ T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The right upper panels show the percentage (mean ± standard deviation) of CD4+ and CD8+ T cells producing simultaneously IL-17 and tumor necrosis factor-alpha (TNF-α) (within IL-17+CD4+ T cells and IL-17+CD8+ T cells, respectively) and amount of protein expressed per cell, measured as MFI (MFI, mean ± standard deviation), after MNC stimulation with PMA plus ionomycin in the absence of MSCs (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4+ and CD8+ T cells expressing IL-17 or IL-17 and TNF-α. Statistically significant differences were considered when P <0.05 for Wilcoxon paired-sample test: *versus MSCs + MNCs + PMA + ionomycin. CM, central memory; EM, effector memory.

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