Figure 6

mRNA expression of interleukin-10 (IL-10), transforming growth factor-beta (TGF-β), IL-2, IL-4, and CTLA-4 in CD4 ⁺ and CD8 ⁺ T cells. The figure shows the results of a semi-quantitative analysis of IL-10, TGF-β, IL-2, IL-4, and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) mRNA expression in fluorescence-activated cell sorting (FACS)-purified CD4⁺ and CD8⁺ T cells from the following culture conditions: non-stimulated mononuclear cells (MNCs), non-stimulated MNCs co-cultured with mesenchymal stromal cells (MSCs) (MNCs + MSCs), MNCs stimulated with phorbol myristate acetate (PMA) plus ionomycin (MNCs + PMA + ionomycin), and MNCs stimulated with PMA plus ionomycin in co-culture with MSCs (MNCs + MSCs + PMA + ionomycin). Statistically significant differences were considered when P <0.05 for Wilcoxon paired-sample test, comparing MNCs + PMA + ionomycin versus MNCs + MSCs + PMA + ionomycin.