Stem Cell Research & Therapy

Table 1 Panel of monoclonal antibody reagents (with clones and commercial source) used for the immunophenotypic characterization of mesenchymal stromal cells and peripheral blood T cells

From: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

Fluorochrome
Tube	PacB	PacO	FITC	PE	PerCPCy5.5 or PECy5	PECy7	APC	APCH7
1	CD44	CD45	CD106	CD73	CD184	CD13	CD90
1	Clone IM7 Biolegend	HI30 Invitrogen	51-10C9 BD Pharmingen	AD2 BD Pharmingen	12G5 BD	Immu103.44 Beckman Coulter	5E10 BD Pharmingen
2	CD3		cyTNF-α	cyIL-17	CD27	CD4	CD45RA	CD8
2	UCHT1 BD Pharmingen		MP6-XT22 BD Pharmingen	SCPL1362 BD Pharmingen	14CD27 Beckman Coulter	SFCI12T4D11 Beckman Coulter	HI100 BD	SK1 BD
3	CD3		cyIFNγ	cyIL-6	CD27	CD4	CD45RA	CD8
3	UCHT1 BD Pharmingen		4S.B3 BD Pharmingen	MQ2-6A3 BD Pharmingen	14CD27 Beckman Coulter	SFCI12T4D11 Beckman Coulter	HI100 BD	SK1 BD
4	CD3		cyIL-2	cyIL-9	CD27	CD4	CD45RA	CD8
4	UCHT1 BD Pharmingen		MQ1-17H12 BD	MH9A3 BD Pharmingen	14CD27 Beckman Coulter	SFCI12T4D11 Beckman Coulter	HI100 BD	SK1 BD
5	CD4		CD25	CD127		TCRγδ	CD8	CD3
5	RPA-T4 BD Pharmingen		M-A251 BD Pharmingen	R34.34 Beckman Coulter		11 F2 BD Pharmingen	B9.11 Beckman Coulter	SK7 BD Pharmingen

Commercial sources: Biolegend (San Diego, CA, USA), Invitrogen, Life Technologies (Carlsbad, CA, USA), BD Pharmingen (San Diego, CA, USA), BD (Becton Dickinson Biosciences, San Jose, CA, USA), Beckman Coulter (Miami, FL, USA). APC, allophycocyanin; APCH7, allophycocyanin-hilite 7; FITC, fluorescein isothiocyanate; mAb, monoclonal antibody; MSC, mesenchymal stromal cell; PacB, pacific blue; PacO, pacific orange; PE, phycoerythrin; PECy5, phycoerythrin-cyanine 5; PECy7, phycoerythrin-cyanine 7; PerCPCy5.5, peridinin chlorophyll protein-cyanine 5.5.

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ISSN: 1757-6512

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