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Table 1 Panel of monoclonal antibody reagents (with clones and commercial source) used for the immunophenotypic characterization of mesenchymal stromal cells and peripheral blood T cells

From: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

Fluorochrome
Tube PacB PacO FITC PE PerCPCy5.5 or PECy5 PECy7 APC APCH7
1 CD44 CD45 CD106 CD73 CD184 CD13 CD90  
Clone IM7 Biolegend HI30 Invitrogen 51-10C9 BD Pharmingen AD2 BD Pharmingen 12G5 BD Immu103.44 Beckman Coulter 5E10 BD Pharmingen  
2 CD3   cyTNF-α cyIL-17 CD27 CD4 CD45RA CD8
UCHT1 BD Pharmingen   MP6-XT22 BD Pharmingen SCPL1362 BD Pharmingen 14CD27 Beckman Coulter SFCI12T4D11 Beckman Coulter HI100 BD SK1 BD
3 CD3   cyIFNγ cyIL-6 CD27 CD4 CD45RA CD8
UCHT1 BD Pharmingen   4S.B3 BD Pharmingen MQ2-6A3 BD Pharmingen 14CD27 Beckman Coulter SFCI12T4D11 Beckman Coulter HI100 BD SK1 BD
4 CD3   cyIL-2 cyIL-9 CD27 CD4 CD45RA CD8
UCHT1 BD Pharmingen   MQ1-17H12 BD MH9A3 BD Pharmingen 14CD27 Beckman Coulter SFCI12T4D11 Beckman Coulter HI100 BD SK1 BD
5 CD4   CD25 CD127   TCRγδ CD8 CD3
RPA-T4 BD Pharmingen   M-A251 BD Pharmingen R34.34 Beckman Coulter   11 F2 BD Pharmingen B9.11 Beckman Coulter SK7 BD Pharmingen
  1. Commercial sources: Biolegend (San Diego, CA, USA), Invitrogen, Life Technologies (Carlsbad, CA, USA), BD Pharmingen (San Diego, CA, USA), BD (Becton Dickinson Biosciences, San Jose, CA, USA), Beckman Coulter (Miami, FL, USA). APC, allophycocyanin; APCH7, allophycocyanin-hilite 7; FITC, fluorescein isothiocyanate; mAb, monoclonal antibody; MSC, mesenchymal stromal cell; PacB, pacific blue; PacO, pacific orange; PE, phycoerythrin; PECy5, phycoerythrin-cyanine 5; PECy7, phycoerythrin-cyanine 7; PerCPCy5.5, peridinin chlorophyll protein-cyanine 5.5.