Kinetics of the epithelial-to-mesenchymal transition during mesenchymal stem cell derivation. (A) mRNA levels of the pluripotent markers OCT-4 and NANOG along the PD-MSCPL derivation protocol, assessed by quantitative PCR. (B) mRNA levels of the epithelial-to-mesenchymal transition markers Snai-2 and Zeb-2, the mesoderm marker T/Brachyury, and the mesenchymal marker Vimentin along the mesenchymal stem cell (MSC) derivation protocol, assessed by quantitative PCR. (C) Evolution of CD90/TRA-1-60, CD73/CD105 and CD172a/CD49d during the PD-MSCPL derivation protocol, assayed by flow cytometry. These data point to a slow transformation of pluripotent stem cells into MSC, taking approximately 2 to 3 weeks to complete. PD-MSCPL, pluripotent-derived mesenchymal stem cells supplemented with platelet lysate.