Figure 5
Pluripotent-derived mesenchymal stem cells supplemented with platelet lysate are fully functional. (A) Optical microcopy of PD-MSCPL differentiated to an adipogenic (a), chondrogenic (b) or osteogenic (c) lineage. (B) Immunomodulation assay. Stimulated peripheral blood mononuclear cells (PBMC) stained with carboxyfluorescein succinimidyl ester (CFSE) show normal proliferation in the absence of mesenchymal stem cells (MSC) (a, left panel) but a reduced proliferation of activated PBMC when cocultured with PD-MSCPL (a, middle panel) compared with that of nonactivated PBMC (a, right panel) in a representative experiment. When stained for CD4 and CD8 lymphocyte populations, a similar finding is seen. Statistical analysis shows significant difference in proliferation from the third doubling onwards (**P <0.1; *P <0.05). PD-MSCPL, pluripotent-derived mesenchymal stem cells supplemented with platelet lysate.