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Table 1 Comparative cell surface expression pattern of the different mesenchymal stem cells analyzed, fibroblasts and pluripotent stem cells

From: A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement

   Fibroblasts PSC PD-MSC PL PD-MSC FBS UC-MSC
Mesenchymal stem cell markers CD90 +++ +++ +++ +++ +++
  CD73 ++ +++ +++ +++
  CD105 ++ +++ +++ +++
  CD166 +++ +++ +++ +++
  CD172alpha + + + ++
  CD271 + + + +
Pluripotent stem cell markers Tra-1-60 +++
  CD133 +++
  CD309 ++
Integrins CD29 +++ +++ +++ +++ +++
  CD49a +++ +++ + ++
  CD49d +++ +++ +++ +++
  CD49e +++ +++ +++ +++ +++
  CD49f +++ +++ +++ +++ +++
  CD51/61 +++ +++ +++ +++
  1. Markers are grouped according to phenotype. No major difference was found between fibroblasts, PD-MSCPL, PD-MSCFBS and UC-MSC. Some markers, however, are useful to distinguish between PSC and mesenchymal cells. –, no expression; +, mild expression; ++, medium expression; +++, strong expression. PD-MSCFBS, pluripotent-derived mesenchymal stem cells supplemented with fetal bovine serum; PD-MSCPL, pluripotent-derived mesenchymal stem cells supplemented with platelet lysate; PSC, pluripotent stem cells; UC-MSC, umbilical cord mesenchymal stem cells.