Table 1 Primers used for quantitative reverse-transcriptase polymerase chain reaction
Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
h-Nanog | TGAACCTCAGCTACAAACAG | TGGTGGTAGGAAGAGTAAAG |
h-Oct4 | CCTCACTTCACTGCACTGTA | CAGGTTTTCTTTCCCTAGCT |
h-Msx1 | CGAGAGGACCCCGTGGATGCAGAG | GGCGGCCATCTTCAGCTTCTCCAG |
h-VEGFA | CGCTCGGTGCTGGAATTTGA | AGTGGGGAATGGCAAGCAAA |
h-VEGFB | TGGAAGAGGAGACTGGGAGG | TAGTGAGGGGAGGAAGAGCC |
h-HIF-1α | CCATGTGACCATGAGGAAAT | CGGCTAGTTAGGGTACACTT |
h-PGF | AGATGAAGCCGGAAAGGTGC | TAAATACACGAGCCGGGTGC |
h-bFGF | CAATTCCCATGTGCTGTGAC | ACCTTGACCTCTCAGCCTCA |
h-TGFB1 | TTGAGGGCTTTCGCCTTAGC | TGAACCCTGCGTTGATGTCC |
h-Angiogenin | CTCGCTTCGGCAGCACA | GGTGGTCGGAGATTCGTAGC |
h-KDR | GTGATCGGAAATGACACTGGAG | CATGTTGGTCACTAACAGAAGCA |
h-bFGFR | GACGGCTCCTACCTCAA | GCTGTAGCCCATGGTGTTG |
h-GAPDH | ATCCCATCACCATCTTCC | GAGTCCTTCCACGATACCA |