Table 1 Primers used for PCR in this study

ACVR1a

ACVR1a-F

TAA GCG TCA CAC TGC CAA AG

Real-time

60

40

89

ACVR1a-R

GTC ACT GGG GTA CTC GGA GA

ACVR1b

ACVR1b-F

TGC AAC AGG ATC GAC TTG AG

Real-time

60

40

121

ACVR1b-R

TGA GGA CAG GAG GAA CAC C

ACVR1c

ACVR1c-F

TGG TCT GGC ACA CCT TCA TA

Real-time

60

40

137

ACVR1c-R

TCA CAG CCA ACC CTA AGT CC

ACVR2a

ACVR2a-F

GCG TTT GCC GTC TTT CTT AT

Real-time

60

40

138

ACVR2a-R

TGT CAC CAT AAC ACG GTT CAA

ACVR2b

ACVR2b-F

CTC CTC TGG GGA TCG CTG T

Real-time

60

40

137

ACVR2b-R

CTT GTC CTG CTC GCC TTC

Brachyury

Brachyury-F

GAA CCA GCA CCC TGT GTC CAC

Standard

55

38

608

Brachyury-R

GCC ACG ACA AAA AGT CAC TGC

CFTR

CFTR-F

ACT TTA AAG CTG TCA AGC CGT G

Standard

35

30

1051

CFTR-R

CAT CAT AGG AAA CAC CAA A

CFTR (nested)

CFTR-NF

AGC TGT CAA GCC GTG TTC TAG ATA

Nested

45

30

140

CFTR-NR

ATG AGG AGT GCC ACT TGC AAA

FOXA2

FOXA2-F

GGG AGC GGT GAA GAT GGA

Standard

55

35

89

FOXA2-R

TCA TGT TGC TCA CGG AGG AGT A

GAPDH

GAPDH-F

ATG GAG AAG GCT GGG GCT C

Real-time and standard

60

40

196

GAPDH-R

AAG TTG TCA TGG ATG ACC TTG

MixL1

MixL1-F

CCG AGT CCA GGA TCC AGG TA

Standard

55

35

58

MixL1-R

CTC TGA CGC CGA GAC TTG G

Nestin

Nestin-F

CAG CTG GCG CAC CTC AAG ATG

Standard

55

35

209

Nestin-R

AGG GAA GTT GGG CTC AGG ACT GG

SPC

SPC-F

AGC CAG AAA CAC ACG GAG AT

Standard

57

30

456

SPC-R

AGT GGA GCC GAT GGA GAA G

SPC (nested)

SPC-NF

AAC GCC TTC TTA TCG TGG TG

Nested

57

30

313

SPC-NR

GTG AGA GCC TCA AGA CTG G

Sox17

Sox17-F

GGC GCA GCA GAA TCC AGA

Real-time

60

40

61

Sox17-R

CCA CGA CTT GCC CAG CAT

TTF1

TTF1-F

ACC AGG ACA CCA TGA GGA AC

Standard

50

38

116

TTF1-R

GCT CAT GTT CAT GCC GCT