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Table 1 Immunostaining protocols

From: Switching of mesodermal and endodermal properties in hTERT-modified and expanded fetal human pancreatic progenitor cells

Antigen

Blocking

Primary Antibody

Dilution

Secondary Antibody

Dilution

Insulin

3% Goat serum

AB3440, Guinea Pig anti-human insulin (Chemicon El Segundo, CA)

1:100

Rhodamine-conjugated anti-guinea pig IgG (AB7136, Abcam, Cambridge, MA)

1:500

Glucagon

3% Goat serum

AB932, Rabbit anti-Glucagon (Chemicon El Segundo, CA)

1:100

Goat anti-Rabbit IgG, Cy3-conjugated (AP132C, Chemicon)

1:500

C-peptide

3% Goat serum

ab14181, Rabbit anti-c-peptide (Abcam Inc, Cambridge, MA)

1:100

Goat anti-Rabbit IgG, Cy3-conjugated (AP132C, Chemicon)

1:500

Vimentin

5% Donkey serum

V212210, (United States Biologicals, Swampscott, MA)

1:100

Rhodamine-conjugated anti mouse IgG (#715-295-150, Jackson Immuno Research)

1:500

  1. IgG, Immunoglobulin G.