Antigen | Blocking | Primary Antibody | Dilution | Secondary Antibody | Dilution |
---|---|---|---|---|---|
Insulin | 3% Goat serum | AB3440, Guinea Pig anti-human insulin (Chemicon El Segundo, CA) | 1:100 | Rhodamine-conjugated anti-guinea pig IgG (AB7136, Abcam, Cambridge, MA) | 1:500 |
Glucagon | 3% Goat serum | AB932, Rabbit anti-Glucagon (Chemicon El Segundo, CA) | 1:100 | Goat anti-Rabbit IgG, Cy3-conjugated (AP132C, Chemicon) | 1:500 |
C-peptide | 3% Goat serum | ab14181, Rabbit anti-c-peptide (Abcam Inc, Cambridge, MA) | 1:100 | Goat anti-Rabbit IgG, Cy3-conjugated (AP132C, Chemicon) | 1:500 |
Vimentin | 5% Donkey serum | V212210, (United States Biologicals, Swampscott, MA) | 1:100 | Rhodamine-conjugated anti mouse IgG (#715-295-150, Jackson Immuno Research) | 1:500 |