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Table 1 Immunostaining protocols

From: Switching of mesodermal and endodermal properties in hTERT-modified and expanded fetal human pancreatic progenitor cells

Antigen Blocking Primary Antibody Dilution Secondary Antibody Dilution
Insulin 3% Goat serum AB3440, Guinea Pig anti-human insulin (Chemicon El Segundo, CA) 1:100 Rhodamine-conjugated anti-guinea pig IgG (AB7136, Abcam, Cambridge, MA) 1:500
Glucagon 3% Goat serum AB932, Rabbit anti-Glucagon (Chemicon El Segundo, CA) 1:100 Goat anti-Rabbit IgG, Cy3-conjugated (AP132C, Chemicon) 1:500
C-peptide 3% Goat serum ab14181, Rabbit anti-c-peptide (Abcam Inc, Cambridge, MA) 1:100 Goat anti-Rabbit IgG, Cy3-conjugated (AP132C, Chemicon) 1:500
Vimentin 5% Donkey serum V212210, (United States Biologicals, Swampscott, MA) 1:100 Rhodamine-conjugated anti mouse IgG (#715-295-150, Jackson Immuno Research) 1:500
  1. IgG, Immunoglobulin G.