Table 2 RT-PCR Primers
Gene | Primer sequences (5'-3'): Forward and Reverse | Tm (°C) | Product (basepairs) | References |
|---|---|---|---|---|
Insulin | GCTGCATCAGAAGAGGCCATCAGGC GCGTCTAGTTGCAGTAGTTCTCCAG | 58 | 380 | [11] |
Glucagon | GAATTCATTGCTTGGCTGGTGAAAGGC CATTTCAAACATCCCACGTGGCATGCA | 60 | 255 | [11] |
Pancreatic Polypeptide | CTGCTGCTGCTGTCCACCTGCGTG CTCCGAGAAGGCCAGCGTGTCCTC | 60 | 206 | [11] |
Somatostatin | CGTCAGTTTCTGCAGAAGTCCCTGGCT CCATAGCCGGGTTTGAGTTAGCAGATC | 60 | 206 | [11] |
NeuroD | ATCCCAACCCACCACCAACC CAGCGGTGCCTGAGAAGATT | 60 | 440 | [15] |
Pancreatic duodenal homeobox1 gene (Pdx1) | CTGCCTTTCCCATGGATGAA CGCTTCTTGTCCTCCTCCTTT | 58 | 277 | [15] |
Prohormone convertase (PC1/3) | TTGGCTGAAAGAGAACGGGATACATCT ACTTCTTTGGTCATTGCTTTGGCGGTG | 65 | 456 | [11] |
Prohormone convertase (PC2) | GCATCAAGCACAGACCTACACTGG GAGACACAAACCACCCTTCATCCTTC | 60 | 308 | [11] |
Chromogranin-A | CGGACAGTTCCATGAAGCTCTC GAGTCAGGAGTAGGAGACAAGG | 58 | 444 | [11] |
Glucokinase | GACGAGTTCCTGCTGGAGTATGAC GACTCGATGAAGGTGATCTCGCAGCTG | 65 | 523 | [11] |
Paired homeobox gene4 (PAX4) | CACCTCTCTGCCTGAGGACACGGTGAG CTGCCTCATTCCAAGCCATACAGTAGTG | 64 | 443 | [11] |
Paired homeobox gene 6 (PAX6) | CAGTCACAGCGGAGTGAATCAGC GCCATCTTGCGTAGGTTGCCCTG | 58 | 519 | [11] |
Glucose transporter2 (GLUT2) | GCCATCCTTCAGTCTCTGCTACTC GCTATCATGCTCACATAACTCATCCA | 65 | 523 | [11] |
Betacellulin2 (BETA2) | CCTGAGCAGAACCAGGACATGCC ATCAAAGGAAGGGCTGGTGCAATCA | 58 | 221 | [11] |
Neurogenin3 (NGN3) | ACTGAGCAAGCAGCGACGGAGTC GCACCCACAGCCGAGCGACAGAC | 65 | 448 | [11] |
NK homeobox protein 6.1 (NKX6 1) | CTCCTCCTCGTCCTCGTCGTCGTC CTTGACCTGACTCTCTGTCATC | 60 | 699 | [11] |
NK homeobox protein 2.2 (NKX2 2) | CGGACAATGACAAGGAGACCCCG CGCTCACCAAGTCCACTGCTGCTGG | 65 | 490 | [11] |
Islet1 (ISL1) | GTGCGGAGTGTAATCAGTATTTGG GTCATCTCTACCAGTTGCTCCTTC | 58 | 519 | [11] |
GATA2 transcription factor | CGTCTTCTTCAATCACCTCG CGTCTTGGAGAAGGGGCTC | 55 | 225 | [10] |
GATA6 transcription factor | GAAGAAGCACATGATTTTGGAC GATGGAAGGGAAGGGCCAG | 58 | 181 | [10] |
Transforming growth factor alpha (TGFα) | ATGGTCCCCTCGGCTGGA GGCCTGCTTCTTCTGGCTGGCA | 58 | 297 | [10] |
Transforming growth factor beta1 (TGFβ1) | GCCCTGGACACCAACTATTGCT AGGCTCCAAATGTAGGGGCAGG | 58 | 161 | [10] |
Transforming growth factor beta2 (TGFβ2) | GATTTCCATCTACAAGACCACGAGGGACTTGC CAGCATCAGTTACATCGAAGGAGAGCCATTCG | 65 | 503 | [10] |
Transforming growth factor beta1 receptor (TGFβ1R) | CGTGCTGACATCTATGCAAT AGCTGCTCCATTGGCATAC | 54 | 251 | [10] |
Transforming growth factor beta2 receptor (TGFβ2R) | TGCACATCGTCCTGTGGAC GTCTCAAACTGCTCTGAAGTGTTC | 58 | 784 | [10] |
Insulin-like growth factor receptor (IGFR) | ACCCGGAGTACTTCAGCGCT CACAGAAGCTTCGTTGAGAA | 55 | 229 | [10] |
Cytokeratin-19 | ATGGCCGAGCAGAACCGGAA CCATGAGCCGCTGGTACTCC | 60 | 308 | [16] |
α-Smooth muscle actin | AGTACCCGATAGAACATGG TTTTCTCCCGGTTGGC | 60 | 153 | [9] |
Vimentin | CACCTACAGCCTCTACG AGCGGTCATTCAGCTC | 60 | 170 | [9] |
Human telomerase reverse transcriptase (hTERT) | AAGTTCCTGCACTGGCTGATGAG TCGTAGTTGAGCACGCTGAACAG | 60 | 378 | [17] |
β-actin | AGAGCTATGAGCTGCCTGAC CTGATCCACATCTGCTGGAA | 55 | 361 | [15] |
Glyceraldehyde phosphate dehydrogenase (GAPDH) | CCATGGAGAAGGCTGGGG CAAAGTTGTCATGGATGACC | 58 | 194 | [10] |