hMSCs suppress lung inflammation regardless of route of administration. A total of 5 × 105 hMSCs were administered OA, IV or IP four hours after LPS exposure. (a) The percentage of delivered hMSCs in the lung after one hour was measured by qRT-PCR of human GAPDH mRNA. (b) Total cell counts in BAL fluid were significantly decreased by hMSC administration via all three routes. (c) Total protein in BAL fluid was slightly decreased by IP delivered MSCs and significantly decreased by both OA and IV delivered MSCs. Data are expressed as mean ± SEM (n = 5 to 6 per group), *P < 0.05 compared with LPS + PBS.