Figure 7

Enhanced levels of ADAM10 in the subventricular zone (SVZ) of adult mice. (a) Expression of the α-secretase ADAM10 is high in the SVZ compared with the hippocampus of APP(+/+) and APP(+/-) mice, suggesting a correlation with sAPPα levels. (b) Quantification of four Western blots showing higher ADAM10 levels in the SVZ compared with the hippocampus. (c) Quantification of four Western blots showing that ADAM10 expression is higher in APP(+/+) SVZ compared with that of the APP(-/-). (d) Representative images of ADAM10 colocalized with Sox2 and nestin in the adult SVZ. Scale bar = 125 μm. (e) Representative images of ADAM10 colocalized with BrdU after a single injection 24 hours prior to sacrifice. Error bars represent standard error of the mean. *P < 0.001 (b), *P < 0.01 (c), Student t test. Scale bar = 250 μm. ADAM, a disintegrin and metalloproteinase; APP, amyloid precursor protein; LV, lateral ventricle; sAPPα, soluble amyloid precursor protein alpha.