Figure 8

Evaluation of miRNA influence on mRNA levels in adipose stem cells (ASCs) from old and young donors. (a) The plot demonstrates significant up- and downregulated mRNA in ACSs from old versus young donors. (b) Fold regulation of significant mRNA assessed in ACSs from old versus young donors. (c) Heatmap shows old versus young donor mRNA expression levels; green represents downregulation of mRNAs, and red represents upregulation of mRNAs. (d) The plot shows the top canonic pathways generated from Ingenuity Pathways Assessment (IPA) analysis of mRNA differences in ACSs from old versus young donors. Yellow line, P-value cutoff (P < 0.05), and yellow dots, ratio of projected involvement of targets to actual inputted involvement. Height of bars is determined by projected involvement of particular pathway. (e) The plot shows top predicted biologic functions from the mRNA data inputted into IPA. Yellow line, P-value cutoff (P < 0.05). (f through i) The top four networks involved in mRNA differences between ACSs from old versus young donors. White items, indirectly involved molecules, those commonly associated with the genes/pathways demonstrated. Green items, downregulated molecules directly entered into IPA. Red items, upregulated molecules based on fold change of mRNA.