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Figure 9 | Stem Cell Research & Therapy

Figure 9

From: MicroRNA profiling reveals age-dependent differential expression of nuclear factor κB and mitogen-activated protein kinase in adipose and bone marrow-derived human mesenchymal stem cells

Figure 9

Western blot analysis of differences in protein levels resulting from donor age and miRNA influences. (a) Immunocytochemical localization of p65 and p50 subunits of NF-κB in representative ASCs from old and young donors. Images are at ×20 magnification. Negative controls are representative images per age group. Images are at ×5 magnification. (b) Representative Western blots of NF-κB pathway elements in ASCs from young (Y) and old (O) donors. (c) Representative Western blots of MAPK/ERK pathway elements in ASCs from young (Y) and old (O) donors. (d) The quantitative fold change in expression of NF-κB pathway proteins normalized to total actin levels. (e) The quantitative fold change in MAPK signaling expression of proteins normalized to total actin levels. Bars, mean ± SEM of at least three Western blot quantifications. *P < 0.05; **P < 0.01; ***P < 0.001).

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