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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Interleukin-1β and transforming growth factor-β cooperate to induce neurosphere formation and increase tumorigenicity of adherent LN-229 glioma cells

Figure 2

IL-1β/TGF-β-induced neurosphere cells express stem cell markers. (A) Transcription levels of stemness genes in control and IL-1β/TGF-β-induced neurospheres. LN-229 cells were cultured in SFM with or without IL-1β/TGF-β for seven days. Differential mRNA levels of stem cell markers were determined by qRT-PCR. β-actin was used as an internal normalization control. Error bars represent SEM. *P < 0.01. (B) Immunoblot analysis for Bmi-1 on cell lysates from control monolayer cells and IL-1β/TGF-β-induced neurospheres. β-actin was used as an internal normalization control. (C) IL-1β/TGF-β-induced neurospheres express nestin, as demonstrated using an immunostaining assay. LN-229 cells were cultured in SFM with or without IL-1β/TGF-β for seven days and stained with anti-nestin antibody. Nuclei (blue stain) were counterstained with DAPI. Scale bar = 20 μm.

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