Representative flow cytometry analysis of BM-derived MSCs cultured in different expansion media. Comparison of membrane antigen expression of MSCs cultured at passage 2 (P2) in four different media: M1 (10% FBS + FGF2) (n = 4), M2 (10% FBS + 5% HPL) (n = 5), M3 (10% HPL) (n = 6), and M4 (5% HPL) (n = 5). The histogram plots represent flow cytometry analysis of the cells with directly labeled monoclonal antibodies (histograms colored inside) or exposed to isotype-matched non-immune directly labeled immunoglobulins (IgG1FITC, IgG1PE, and IgG1PerCP). MSCs were negative for hematopoietic markers CD45, CD34, CD133, and CD14 (A) but were positive for CD90, CD73, CD105, and CD106 and weakly positive for CD49e (B). (A) Hematopoietic and immaturity markers. (B) Stromal cell markers.