Western blot analysis showing differentiation markers of osteogenic, vascular smooth muscle, and adipogenic lineages expressed by BM-derived MSCs cultured in different expansion media. The media are M1 (10% FBS + FGF2), M2 (10% FBS + 5% HPL), M3 (10% HPL), and M4 (5% HPL). The experiments were performed in triplicate, and one representative blot is shown here. Beta-actin expression was used as positive control. Osteogenic proteins studied are calcium-sensing receptor (CaSR) and parathormone receptor (PTHR). Adipogenic protein studied is Leptin. Vascular smooth muscle proteins studied are smooth muscle 22alpha (SM22α) and alpha-smooth muscle actin (ASMA). D0, day 0 (before induction); D14, day 14 (after induction).