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Table 1 Sequences of the primers used in reverse transcription-polymerase chain reaction study

From: Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum

Construct Forward sequence Reverse sequence
GAPDH 5'- AATCCCATCACCATCTTCCAGG-3' 5'-AGAGGCAGGGATGATGTTCTGG-3'
ALP 5'-CTGGACCTCGTTGACACCTG-3' 5'-GACATTCTCTCGTTCACCGC-3'
RUNX2 5'-AACTTCCTGTGCTCGGTGCTG-3' 5'-GGGGAGGATTTGTGAAGACGG-3'
LPL 5'-AAAGCCCTGCTCGTGCTGAC-3' 5'-TAAACCGGGCCACATCCTGT-3'
PPARĪ³ 5'-GGAGAAGCTGTTGGCGGAGA-3' 5'-TCAAGGAGGCCAGCATTGTG-3'
ASMA 5'-TCATGATGCTGTTGTAGGTGGT-3' 5'-CTGTTCCAGCCATCCTTCAT-3'
  1. ALP, alkaline phosphatase; ASMA, alpha smooth muscle actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LPL, lipoprotein lipase; PPARĪ³, peroxisome proliferator-activated receptor-gamma; RUNX2, Runt-related transcription factor 2.