Skip to main content

Table 1 Sequences of the primers used in reverse transcription-polymerase chain reaction study

From: Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum

Construct

Forward sequence

Reverse sequence

GAPDH

5'- AATCCCATCACCATCTTCCAGG-3'

5'-AGAGGCAGGGATGATGTTCTGG-3'

ALP

5'-CTGGACCTCGTTGACACCTG-3'

5'-GACATTCTCTCGTTCACCGC-3'

RUNX2

5'-AACTTCCTGTGCTCGGTGCTG-3'

5'-GGGGAGGATTTGTGAAGACGG-3'

LPL

5'-AAAGCCCTGCTCGTGCTGAC-3'

5'-TAAACCGGGCCACATCCTGT-3'

PPARγ

5'-GGAGAAGCTGTTGGCGGAGA-3'

5'-TCAAGGAGGCCAGCATTGTG-3'

ASMA

5'-TCATGATGCTGTTGTAGGTGGT-3'

5'-CTGTTCCAGCCATCCTTCAT-3'

  1. ALP, alkaline phosphatase; ASMA, alpha smooth muscle actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LPL, lipoprotein lipase; PPARγ, peroxisome proliferator-activated receptor-gamma; RUNX2, Runt-related transcription factor 2.
Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512