Platelet-derived circulating soluble P-selectin is sufficient to induce hematopoietic stem cell mobilization

Background Granulocyte colony-stimulating factor (G-CSF)-mediated mobilization of hematopoietic stem cells (HSCs) is a well-established method to prepare HSCs for transplantation nowadays. A sufficient number of HSCs is critical for successful HSC transplantation. However, approximately 2–6% of healthy stem cell donors are G-CSF-poor mobilizers for unknown reasons; thus increasing the uncertainties of HSC transplantation. The mechanism underlining G-CSF-mediated HSC mobilization remains elusive, so detailed mechanisms and an enhanced HSC mobilization strategy are urgently needed. Evidence suggests that P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) are one of the cell–cell adhesion ligand–receptor pairs for HSCs to keep contacting bone marrow (BM) stromal cells before being mobilized into circulation. This study hypothesized that blockage of PSGL-1 and P-selectin may disrupt HSC-stromal cell interaction and facilitate HSC mobilization. Methods The plasma levels of soluble P-selectin (sP-sel) before and after G-CSF administration in humans and male C57BL/6J mice were analyzed using enzyme-linked immunosorbent assay. Male mice with P-selectin deficiency (Selp−/−) were further employed to investigate whether P-selectin is essential for G-CSF-induced HSC mobilization and determine which cell lineage is sP-sel derived from. Finally, wild-type mice were injected with either G-CSF or recombinant sP-sel to investigate whether sP-sel alone is sufficient for inducing HSC mobilization and whether it accomplishes this by binding to HSCs and disrupting their interaction with stromal cells in the BM. Results A significant increase in plasma sP-sel levels was observed in humans and mice following G-CSF administration. Treatments of G-CSF induced a decrease in the level of HSC mobilization in Selp−/− mice compared with the wild-type (Selp+/+) controls. Additionally, the transfer of platelets derived from wild-type mice can ameliorate the defected HSC mobilization in the Selp−/− recipients. G-CSF induces the release of sP-sel from platelets, which is sufficient to mobilize BM HSCs into the circulation of mice by disrupting the PSGL-1 and P-selectin interaction between HSCs and stromal cells. These results collectively suggested that P-selectin is a critical factor for G-CSF-induced HSC mobilization. Conclusions sP-sel was identified as a novel endogenous HSC-mobilizing agent. sP-sel injections achieved a relatively faster and more convenient regimen to mobilize HSCs in mice than G-CSF. These findings may serve as a reference for developing and optimizing human HSC mobilization in the future. Supplementary Information The online version contains supplementary material available at 10.1186/s13287-023-03527-w.

Conclusions sP-sel was identified as a novel endogenous HSC-mobilizing agent.sP-sel injections achieved a relatively faster and more convenient regimen to mobilize HSCs in mice than G-CSF.These findings may serve as a reference for developing and optimizing human HSC mobilization in the future.Keywords Granulocyte colony-stimulating factor, Hematopoietic stem cell mobilization, Soluble P-selectin

Background
Hematopoietic stem cells (HSCs) exert the ability to selfrenew and differentiate into all kinds of hematopoietic cells (red blood cells, white blood cells, and platelets) in human body [1].HSC transplantation has been used to treat hematological diseases for over 50 years [2,3].Transplantation with granulocyte colony-stimulating factor (G-CSF)-mobilized HSCs is more convenient and popular than transplantation using bone marrow (BM) HSCs because of lower cost, faster engraftment, and shorter hospitalization [4].However, an estimated 2-6% of stem cell donors are poor mobilizers for G-CSF [5][6][7][8].An insufficient number of HSCs could lead to failure of HSC transplantation and mortality of the recipients [9,10], suggesting an urgent need to improve the HSC mobilization regimen.
G-CSF regulates granulopoiesis and prevents neutropenia in patients after chemotherapy; it is now the clinical routinely used inducer to mobilize HSCs for transplantation [16][17][18].However, the detailed mechanism of how G-CSF induces HSC mobilization remains elusive.Reports suggested five non-mutually exclusive but not completely unified mechanisms involved in G-CSF-induced HSC mobilization [19].1. G-CSF activates neutrophil proteases (e.g., matrix metalloproteinases, neutrophil elastase, and cathepsin G) to cleave and inactivate the retention axes [20][21][22][23][24]. 2. G-CSF reduces the expression of SDF-1 on stromal cells by activating the sympathetic neurons or macrophages in BM, resulting in HSC mobilization [25].3. G-CSF activates innate immune cells, activating the inflammasome and releasing interleukin (IL)-1β, IL-18, and danger-associated molecular patterns.These molecules then activate the complement system through the mannan-binding lectin-dependent pathway.Subsequently, sphingosine-1-phosphate (S1P) is released from complement-lysed erythrocytes, serving as a chemoattractant for HSC mobilization [26][27][28][29].4. G-CSF increases the expression and activity of dipeptidyl peptidase 4 (DPP4/CD26) on the surface of endothelial cells.CD26 truncates the neurotransmitter neuropeptide Y (NPY), and the truncated NPY reduces the expression of VE-cadherin and CD31, resulting in HSC trans-endothelial migration [30,31]. 5. Recently, a paper suggested that nociceptive nerves are required for G-CSF-induced HSC mobilization [32].AMD3100 (Plerixafor) is another clinically used inducer to mobilize HSCs.As a CXCR4 antagonist, it can bind to CXCR4 and interfere with one of the retention axes (SDF-1 and CXCR4) [33].Treatments of AMD3100 result in more rapid mobilization of HSCs than that of G-CSF.However, the efficiency of HSC mobilization by AMD3100 alone is much less than that of G-CSF.Therefore, AMD3100 serves as an accessory treatment in combination with G-CSF to induce a more effective mobilization for clinical use, especially in heavily G-CSF-pretreated patients with lymphoma and myeloma [33][34][35].
P-selectin and PSGL-1 are another retention axes to maintain HSCs in BM [12,36].P-selectin is expressed in platelets and endothelial cells [37,38].Soluble P-selectin (sP-sel) can be released into circulation by alternative splicing form lacking the trans-membrane domain or proteolytic cleavage of the ectodomain of P-selectin by matrix metalloproteinase [39][40][41].Increased plasma sPsel can be found in patients with hematological malignancies after G-CSF treatments [42,43].In addition, G-CSF induced myeloid cell mobilization in PSGL-1 deficiency mice [44] and reduced PSGL-1 expression on the surfaces of human neutrophils [45].This evidence suggests that P-selectin plays a critical role in G-CSFinduced HSC mobilization.
In this study, the plasma levels of sP-sel in humans and mice before and after G-CSF administration were analyzed.In the mouse model, the role of P-selectin in G-CSF-induced HSC mobilization was analyzed using the loss-of-function (G-CSF treatments in Selp −/− mice) and gain-of-function approaches (injection of sP-sel in wild-type mice).

Human sample collection
For stem cell mobilization, G-CSF (Filgrastim, Kirin Brewery Co., Tokyo, Japan) was given subcutaneously daily (10 μg/kg) for 5 consecutive days.Residual specimens from clinical peripheral blood examination of stem cell donors after the last dose of G-CSF administration were collected.Peripheral blood (10 mL) was provided by stem cell donors 1-2 months before G-CSF administrations.Peripheral blood was collected via venipuncture using anticoagulant K 2 EDTA-coated purple-top tubes.Peripheral blood (1-5 mL) was twofold diluted with phosphate-buffered saline (PBS) and layered on 5 ml Ficoll-Paque PLUS (GE Healthcare).Plasma fractions were collected after centrifugation (400 × g for 40 min) at room temperature and used for sP-sel detection.

Animals
C57BL/6J mice were purchased from the National Laboratory Animal Center (Taipei, Taiwan).P-selectin deficiency mice (Selp −/− ; B6.129S-Selp tm1Bay /J) were obtained from Jackson Laboratory (Bar Harbor, ME) [46].All animals were maintained in a specific pathogen-free environment in the experimental animal center of Tzu Chi University (Hualien, Taiwan).The experimental protocols involving mice followed the ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments).These protocols were approved by the Institutional Animal Care and Use Committee of Tzu Chi University under approval IDs 104105, 107085-B, and 109073.Before performing retro-orbital injections or withdrawals, a drop of an anesthetic agent, specifically proparacaine hydrochloride (0.5% Alcaine), was gently applied to the mice's eyeballs.This step was taken to minimize discomfort and pain during the procedure [47].After the experiments were concluded, the mice were placed in a euthanasia chamber (five mice per 8.5 L) and euthanized using 100% carbon dioxide at a flow rate of 20-30% of the chamber's volume per minute.This gradual flow rate ensured the loss of consciousness without causing pain [48].Once the animals had lost consciousness, the flow rate could be increased to 40% of the chamber's volume per minute if necessary.The entire process was completed within 5-6 min and adhered to the guidelines established by the Institutional Animal Care and Use Committee of Tzu Chi University.

G-CSF and P-selectin injections in mice
C57BL/6J mice (male, 11-20 weeks of age) and Selp −/− mice (male, 10-19 weeks of age) were subcutaneously injected with 250 μg/kg/day of G-CSF (Filgrastim, in 200 μL normal saline) or the same volume of normal saline for 4 consecutive days.C57BL/6J mice (male, 12-15 weeks of age) were retro-orbitally injected with 0.1, 0.5, or 1 mg/kg of sP-sel [purified mouse P-selectin-IgG fusion protein (BD Pharmingen) or recombinant mouse P-selectin/CD62P Fc chimera (R & D systems) used in Fig. 5, in 200 μL saline] two times a day for 1 day.According to the manufacturer's instructions, the P-selectin-IgG fusion protein comprises the signal sequence, the lectin domain, the epidermal growth factor-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2, and CH3) of human IgG1 (P-selectin-IgG-Fc).The Fc fusion protein was designed to increase the half-life in the circulation without reducing biological efficacy [49,50], and the recombinant protein was purified from tissue culture supernatant or ascites by affinity chromatography.As antibodies (IgGs) can bind to specific blood and immune cells expressing receptors for the Fc portion of antibodies (FcRs) [51,52], the potential involvement of Fc-Fc receptor binding in soluble P-selectin (P-selectin-IgG-Fc)-mediated HSC mobilization was investigated.Human IgG Fc fragment (Jackson ImmunoResearch Laboratories) was injected into mice as a control group at 1 mg/kg two times a day for 1 day.Peripheral blood was retro-orbitally obtained 1 week before injection, on day 2 after the second G-CSF injection, or 17 h after the last G-CSF or sP-sel and IgG Fc injection.Peripheral blood (200 μL) and anticoagulant acid citrate dextrose formula A (ACD-A, 300 μL) were mixed well and loaded on top of 5 mL Ficoll-Paque PLUS.Plasma fraction and mononuclear cell fraction were collected after centrifugation (400 × g for 40 min) at room temperature and used for sP-sel detection and LSK cell analysis, respectively.

Detection of soluble P-selectin in humans and mice
Human and mouse plasma were twofold and 40-fold diluted with PBS, respectively.The levels of human and mouse sP-sel were measured using a custommade ProcartaPlex Immunoassay (Thermo Fisher Scientific, Waltham, MA, USA) and enzyme-linked immunosorbent assay (ELISA) in accordance with the manufacturer's instructions (mouse P-selectin ELISA kit, RayBiotech, Inc., USA), respectively.

Detection of P-selectin in microparticle and soluble fraction
Selp −/− mice (male, 10-19 weeks of age) were retroorbitally injected with platelets from wild-type C57BL/6J mice at 1 × 10 8 per mouse per day.This injection was administered for 3 consecutive days in conjunction with the last three doses of G-CSF (250 μg/kg/ day).The platelet purification and transfer procedures followed the prior study's methods [53].After Ficoll-Paque PLUS purification, the mouse plasma underwent two rounds of centrifugation at room temperature, each at 9170 × g for 10 min, to eliminate any residual platelets.Subsequently, the supernatant underwent ultra-centrifugation at 157,000 × g for 1.5 h at 4 °C to segregate microparticles (in the pellet) from the microparticle-free supernatant [54].The concentrations of P-selectin in microparticle and soluble fraction were determined using the mouse P-selectin ELISA kit (Ray-Biotech, Inc.USA).

Analysis of LSK cells in peripheral blood
Mononuclear cells isolated from the peripheral blood were counted and incubated with 5% bovine serum albumin (Sigma-Aldrich) at 37 °C for 1 h to block nonspecific binding.Then, they were incubated with antibodies against mouse lineage conjugated with fluorescein isothiocyanate (FITC) (BioLegend), mouse Ly-6A/E (stem cell antigen-1, Sca-1) conjugated allophycocyanin (APC) (BioLegend), and mouse CD117 (c-Kit) conjugated with phycoerythrin/Cy7 (PE/Cy7) (BioLegend) or mouse P-selectin conjugated with PE (eBioscience) at 4 °C for 30 min.After the cells were washed with PBS, they were resuspended in 300 μL PBS and analyzed using a flow cytometer (Gallios, Beckman Coulter Life Sciences).

Detection of white blood cell (WBC) in mice
Mouse peripheral blood (200 μL) and anticoagulant ACD-A (300 μL) were mixed well, and then 25 μL was taken and mixed with 75 μL ACD-A.WBC count was measured using an automated hematology analyzer (XP-300, Sysmex Corporation).

Statistics
Data are presented as median (Q1 and Q3) or mean ± standard deviation (SD) depending on their distribution (normal or not).Statistical analyses were conducted on Microsoft Office Excel 2003.Comparisons between groups were conducted using two-tailed Student's t-test.A P value of less than 0.05 was considered statistically significant.Graphs of human samples were created on GraphPad Prism 5 software.The significance of human samples was analyzed by paired t-test or Mann-Whitney U test depending on its distribution (normal or not).G*Power version 3.1.9.2 was used to calculate the mouse sample size needed [55,56].For example, a one-sided test was performed to test the hypothesis that wild-type platelets could ameliorate HSC mobilization defect in Selp −/− mice, with an effect size of 1.66, an α of 0.05, and a power (1-β) of 0.80.The estimated sample size was 12 (i.e., 6 per group).One-sided test was also conducted to examine the hypothesis that the LSK cell counts after 0.1 mg/kg sP-sel injection are less than those after G-CSF treatment.An effect size of 2.97, an α of 0.05, and a power (1-β) of 0.80, and a sample size ratio of 1 were set, and the estimated sample size was 6 (i.e., 3 per group).

Soluble P-selectin levels were positively associated with HSC mobilization and upregulated in plasma after G-CSF treatments
From August 2015 to January 2018, a total of 309 HSC donors were recruited and categorized as good mobilizers (circulating CD34 + cells in peripheral blood ≥ 180/μL, n = 15, 4.85%) and poor mobilizers (CD34 + cells ≤ 25/μL, n = 14, 4.53%) in accordance with the number of CD34 + cells in their peripheral blood after five doses of G-CSF administration [57].Residual specimens from the routine clinical examinations of the donors after the last dose of G-CSF treatments in Hualien Tzu Chi Hospital were collected.Twenty-six soluble proteins related to adhesion molecule (including sP-sel) and inflammatory factors were examined using a custom-made Procarta-Plex Immunoassay (Fig. 1A, experiment outline for post-G-CSF administration) to check whether the levels of soluble factors in plasma are correlated with HSC mobilization.The results revealed that good mobilizers have higher sP-sel in plasma than poor mobilizers (Fig. 1B).Although previous studies suggested that gender and age are associated with HSC mobilization [5,7,[58][59][60][61], the difference remained after adjustment for age and gender by multiple linear regression (Additional file 1: Table S1).Paired samples (before and after G-CSF administration) were obtained from the same donors to examine whether sP-sel is induced by G-CSF.Collecting the paired samples was not easy compared with collecting post-G-CSF samples.Therefore, the paired samples were much less than the post-G-CSF samples (n = 7).The sP-sel levels of the paired plasma samples from the same cohort of stem cell donors before (pretreated) and after G-CSF administration (G-CSF) were analyzed using ELISA (Fig. 1C, experiment outline for the paired samples).Pretreated samples were isolated from stem cell donors 1-2 months before G-CSF administration.The data revealed that compared with those in the pretreated stage, the circulating sP-sel levels were markedly upregulated in the plasma of HSC donors after G-CSF administration (Fig. 1D).The levels of sP-sel were positively associated with HSC mobilization and upregulated in plasma after G-CSF administration in healthy stem-cell donors.

Soluble P-selectin levels were upregulated after G-CSF treatments in mice
Due to time consumption in collecting sufficient clinical paired donors, the convenience of collecting samples during G-CSF administrations, and the possibility of using gene-deficiency mice to confirm the importance of P-selectin in HSC mobilization, a mouse model for G-CSF-induced HSC mobilization was established on the basis of previous studies [62][63][64][65][66].In the present study, male mice were used to rule out the influence of gender in HSC mobilization.C57BL/6 J mice were subcutaneously injected with 250 μg/kg G-CSF daily for 4 consecutive days.Peripheral blood was taken 1 week before the first G-CSF injection, on day 2 (after the second G-CSF injection), and on day 4 (after the last G-CSF injection).Normal saline-treated mice served as negative vehicle controls (Fig. 2A).After the treatments, the number of mouse WBC markedly increased on days 2 and 4 (Fig. 2B).Hematopoietic Lin − Sca-1 + c-Kit + (LSK) cells, which express no lineage-specific markers (e.g., lymphocytes, monocytes/macrophages, granulocytes, natural killer cells, and erythrocytes)-lineage negative (Lin − ), but have Sca-1 and c-kit (stem cell factor receptor or CD117) expressed on their surfaces, are equivalent to human CD34 + HSCs [67,68].They were found to be considerably increased in the circulation after G-CSF administrations (Fig. 2C).By contrast, the normal-saline-treated mice showed no obvious increase in WBC and LSK levels in the circulation (Figs.2B and 2C).In agreement with the human study (Fig. 1B), the established animal model showed upregulated plasma sP-sel levels after G-CSF treatments, similar to humans (Fig. 2D), compared with the normal-saline-treated mice.

G-CSF-induced HSC mobilization was defective in P-selectin deficiency mice
Wild-type and P-selectin deficiency (Selp −/− ) mice were used to characterize whether P-selectin is required for G-CSF-induced HSC mobilization (Fig. 3A).The data indicated that G-CSF can mobilize LSK cells to the peripheral blood in Selp −/− mice.However, the LSK cell number was markedly lower than that in wild-type mice (about 0.68-fold, Fig. 3B).These results suggested that P-selectin is critical for G-CSF-induced HSC mobilization, and sP-sel may be one of the G-CSF-downstream native inducers for conducting HSC mobilization.P-selectin is almost exclusively expressed and stored in the α-granule of platelet and the Weibel-Palade body of endothelial cells [41].Soluble P-selectin can be released into circulation by alternative splicing forms lacking the trans-membrane domain or proteolytic cleavage of the ectodomain [41].Selp −/− mice were injected with G-CSF together with platelets collected from wildtype mice to check whether G-CSF-induced sP-sel is derived from platelet (Fig. 3A).The result indicated that wild-type platelets can ameliorate HSC mobilization defect in Selp −/− mice (Fig. 3B).High-speed centrifugation was applied to remove residual platelets from the plasma to study the influence of G-CSF on sP-sel release from platelets.The plasma was then separated into microparticle and soluble fractions.The data revealed that in the presence of G-CSF administration, the wild-type platelet-transferred-Selp −/− mice exhibited significantly more sP-sel than the G-CSF-treated Selp −/− mice (Fig. 3C).These findings underscored the necessity of G-CSF-induced platelet-derived sP-sel for HSC mobilization.

Soluble P-selectin alone could induce HSC mobilization
All the above data indicated that sP-sel is one of the downstream mediators of G-CSF.Next, whether sP-sel alone is sufficient for inducing HSC mobilization in mice and whether G-CSF and sP-sel synergistically affect HSC mobilization were investigated.Wild-type mice were subcutaneously injected with G-CSF (250 μg/kg/day) for 4 consecutive days.Additionally, they were treated with recombinant sP-sel (0.1, 0.5, or 1 mg/kg) two times daily for 1 day.Another group of mice received combined treatment of G-CSF and sP-sel (0.1 mg/kg, Fig. 4A).Although the LSK cell counts in the group injected with 0.1 mg/kg of sP-sel were lower than those in the G-CSF Fig. 2 Levels of white blood cell numbers, LSK cells, and plasma soluble P-selectin in G-CSF-treated mice.The experimental outline is shown (A).Peripheral blood was collected before and on day 2 and 4 after G-CSF treatments (250 μg/kg/day, n = 6, two experiments with six mice per group).Normal saline-treated mice (n = 4, two experiments with four mice per group) served as negative controls.White blood cell (WBC) numbers (B), LSK (Lin − /Sca-1 + /c-Kit + ) cell numbers (C), and plasma sP-sel (D) were detected using hematology analysis, flow cytometry (FC), and ELISA, respectively.Data are reported as mean ± standard deviation (SD).**P < 0.01 compared with the before experiment groups (Before Exp) treatment group, a significant increase in the number of LSK cells was observed in all groups following treatment (Fig. 4B).The critical finding is that compared with four doses of 4-day treatments of G-CSF, the other two doses (0.5 and 1 mg/kg) of 1-day treatments of sP-sel alone can sufficiently induce comparable LSK cell mobilization (Fig. 4B).The combined treatment of G-CSF and sP-sel resulted in LSK cell counts that were comparable to those observed in G-CSF treatment alone (Fig. 4B).This finding suggested that G-CSF and sP-sel do not have a synergistic  effect on HSC mobilization.Due to the generation of sP-sel through the fusion of human IgG Fc portion to enhance its half-life in circulation, precautions were implemented to eliminate any potential immunological reactions caused by the Fc portion of IgG1 binding to Fc receptors on immune cells [51,52].As part of the negative control, the human IgG Fc portion was injected two times a day for one day (Fig. 4A).The data revealed that the IgG Fc fragment was unable to induce HSC mobilization (Fig. 4B).These findings suggested that the observed HSC mobilization is specifically attributed to soluble P-selectin rather than the IgG Fc component.

Soluble P-selectin mobilized HSCs through disrupting the interaction between HSCs and stromal cells in BM
P-selectin and E-selectin on endothelial cells and P-selectin on megakaryocytes in BM interact with PSGL-1 on HSCs, crucially contributing to HSC retention [69][70][71][72][73].This study hypothesized that sP-sel binds to PSGL-1 on HSCs, disrupting the interactions and facilitating the mobilization of HSCs into the peripheral blood.An experiment was designed to test this hypothesis (Fig. 5A).The results demonstrated that in the G-CSF-and sP-seltreated groups, a comparable mobilization of LSK cells into the peripheral blood was observed (Fig. 5B).However, the percentages of P-sel + cells within the LSK populations significantly increased in the sP-sel administration group (Fig. 5C).This finding strongly supported the notion that sP-sel promotes HSC mobilization by binding to LSK cells and disrupting the interactions between HSCs and stromal cells within BM.

Discussion
Clinical studies showed that circulating sP-sel levels were upregulated after G-CSF administration in pathological conditions, such as hematological malignancies [42,43].However, whether the sP-sel levels also increase after G-CSF administration in healthy stem-cell donors remains uncertain.The present study demonstrated that G-CSF administration induced higher plasma sP-sel levels in healthy humans and a mouse HSC-mobilization model.Moreover, the levels of sP-sel were positively associated with HSC mobilization.In addition, P-selectin deficiency negatively affected G-CSF induced-HSC mobilization, and G-CSF-induced wild-type platelet-derived sP-sel can ameliorate the HSC mobilization defect caused by P-selectin deficiency.Notably, compared with the four administrations of G-CSF (4-day period), only two injections of sP-sel (1-day period) showed similar LSK cell counts in the peripheral blood, suggesting the superior effect of sP-sel over G-CSF on HSC mobilization.In addition, the experiments provided evidence that sP-sel facilitates HSC mobilization by disrupting the crucial interactions between HSCs and stromal cells in BM.
In clinical settings, G-CSF is a routinely used agent for HSC mobilization.However, 5 continuous days are required to proceed with the whole injection process due to the low efficiency and short half-life of G-CSF (about 3-4 h) [74,75].The ideal mobilization regimen should be fast, efficient, and natural with fewer adverse effects and not needing multiple injections over several days.The mouse model in this study demonstrated that a more convenient 1-day injection course may improve such 4-day processes of G-CSF administration through sP-sel administration.Fast HSC mobilization was also demonstrated in recent reports.For example, a single injection of CXC chemokine receptor 2 agonist-growthregulated protein β and AMD3100 and a single oral dose of Viagra combined with a single injection of AMD3100 resulted in rapid HSC mobilization within 15 min and 2 h, respectively [76,77].Although the above two regimens can mobilize HSCs rapidly within a short time course, growth-regulated protein β or Viagra alone is unable to achieve the effect unless combined with AMD3100.However, some recipients who received AMD3100mobilized stem cells have secondary myelodysplastic syndrome/acute myeloid leukemia symptoms [78,79].As a native host factor, sP-sel is highly compatible with the human body.Reports showed upregulated sP-sel levels in the circulation of patients with inflammatory conditions, such as hypertension [39], cardiovascular disease [40], rheumatic mitral stenosis [80], myocardial infarction [81], and sepsis [82].Consistently, animal experiments suggested that the sP-sel levels were upregulated in mice with inflammatory burdens, such as under anthrax lethal toxin [46] and snake venom treatments [83].These studies suggested that sP-sel can be a native factor for conducting anti-inflammatory and repairing effects.In the present study, sP-sel (two injections only in 1 day) can be a native inducer to mobilize HSCs within one day, comparable with G-CSF administration, which typically takes 4 days.Notably, the potential side effects of high sP-sel concentration in the human body should be a concern when the regimen is applied to clinical medicine in the future.
Inhibition of P-selectin and PSGL-1 interaction is considered a feasible strategy to manage severe inflammation, because the interaction of P-selectin/PSGL-1 is a critical step to initiate the binding of circulating leukocytes to the endothelium and infiltrate into the inflamed tissues [84][85][86][87][88][89].Similarly, it has been postulated that the P-selectin-PSGL-1 interaction between HSCs and BM stroma cells is necessary for HSCs retention in BM [12].Accordingly, in the present study, sPsel served as a native competitor and antagonist for disrupting HSC-stromal cell interaction, thus facilitating HSC mobilization.The presence of HSCs in the BM of Selp −/− mice suggested that other HSC-stromal cell interaction machinery supports BM residential HSCs.However, given that the P-selectin defect reduced the G-CSF-mediated HSC mobilization, the P-selectin pathway may play a crucial role in regulating HSC stability and motility in BM.P-selectin is primarily expressed and stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells [41].P-selectin on the surface of endothelial cells in BM also plays a vital role in hematopoietic progenitor cells residing in BM [71,72].The endothelial cells in BM serve as a physical barrier for HSC trafficking and as stromal cells to retain HSCs in BM [30,31,90].Loss of the endothelial transmembrane protein Robo4 or activating vascular endothelial growth factor receptor 2 facilitates HSC mobilization from BM to peripheral blood [91,92], suggesting that the endothelial cells in BM may play a crucial role in HSC mobilization.A notable detail that the P-selectin-deficiency mice were not specifically engineered to have a tissue-specific knockout, so P-selectin deficiency was observed across all P-selectin-expressing cell types, including platelets and endothelial cells in mice.However, the findings demonstrated that the defect in HSC mobilization observed in Selp −/− mice can be fully restored by transferring wildtype platelets.Furthermore, G-CSF stimulates the release of sP-sel from platelets.Considering that a total of 3 × 10 8 wild-type platelets only was transfused into the P-selectin-deficiency mice (with approximately 1 × 10 9 /mL platelets), the quantity of sP-sel released by these platelets may be less than that induced by G-CSF treatment in wild-type mice.However, this soluble form of P-selectin, even when secreted in small amounts by platelets, holds the potential to alleviate the HSC mobilization deficiency observed in mice lacking P-selectin.Investigating whether G-CSF directly or indirectly induces platelets to release sP-sel remains a promising avenue for further research.The data suggested that while endothelial cells may contribute to the process, platelets play a dominant role in G-CSF-induced HSC mobilization.
Although numerous reported mechanisms attempt to explain how G-CSF induces HSC mobilization, they are not mutually exclusive and remain largely ununified thus far.Multiple paired molecules between HSCs and stromal cells play a role in retaining HSCs in BM.In this study, elevated circulating levels of sP-sel disrupted the interaction between PSGL-1 and P-selectin on HSCs and stromal cells, ultimately promoting HSC mobilization.This competitive mechanism closely resembles the action of AMD3100, which binds to CXCR4 expressed on the surface of HSCs and disrupts their interactions with stromal cells that express SDF-1 [33,93].Comparison with the five mechanisms (Introduction section) that are known to mobilize HSCs by G-CSF administration showed that the mechanism of sP-selmediated HSC mobilization is similar to mechanisms 1 and 2, where cleavage or attenuation of retention axes occurs.In mechanism 3, pro-inflammatory factors such as IL-1β and IL-18, are involved in HSC mobilization [26].The authors' previous study found that HSC mobilization following G-CSF administration was linked to pro-inflammatory factors such as IL-22, tumor necrosis factor, and interferon-γ [5].These results align with earlier research on infectious diseases, toxin treatments, and cardiovascular injuries [46,94,95], indicating an association between circulating levels of sP-sel and inflammatory conditions.The previously reported pro-survival and tissue-repair functions of sP-sel [46,83,96] could be partly attributed to HSC mobilization, highlighting a natural self-rescue feedback mechanism between sP-sel-mediated tissue repair and inflammation-related tissue damage.Meanwhile, S1P was shown to facilitate HSC mobilization [97].S1P is a pro-inflammatory factor that promotes inflammation by binding to its receptors on immune cells and inducing their activation, infiltration, and pro-inflammatory cytokines [98].Intriguingly, S1P receptor promotes leukocyte rolling by mobilizing endothelial P-selectin [99], suggesting a regulatory role and crosstalk of S1P pathway on the modulation of endothelial P-selectin expression under inflammation.Regardless of any mechanism, HSCs require mechanism 4 to open the gate of BM and mobilize in peripheral blood.The mechanisms on how G-CSF induces upregulation of sP-sel and any potential crosstalk between other mobilization mechanisms are not yet fully understood, and further investigation is warranted to elucidate the detailed mechanism.

Conclusions
In conclusion, sP-sel was identified as a novel endogenous agent for mobilizing HSCs.sP-sel injections achieved a faster and more convenient regimen for mobilizing HSCs than G-CSF in mice.These findings could offer valuable insights for future endeavors to develop and optimize the mobilization of human HSCs.• thorough peer review by experienced researchers in your field • rapid publication on acceptance • support for research data, including large and complex data types • gold Open Access which fosters wider collaboration and increased citations maximum visibility for your research: over 100M website views per year

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Fig. 1
Fig. 1 Correlation between plasma soluble P-selectin and hematopoietic stem cell mobilization.The experimental outline for measuring plasma sP-sel in stem-cell donors post-G-CSF administration is shown (A).Using ProcartaPlex Immunoassay, sP-sel levels were detected in 15 good and 14 poor mobilizers after 5 consecutive days of G-CSF treatments (B).Each closed circle and closed square represents an individual with good or poor mobilization.The data are presented as median (Q1 and Q3).The experimental outline for measuring plasma sP-sel in stem cell donors before and after G-CSF administrations is shown (C).Using ELISA, the levels of sP-sel were detected in 7 paired donors before and after 5 consecutive days of G-CSF administration (D).The data from the same donor are line-paired.*P < 0.05 and **P < 0.01 compared with the indicated groups.

Fig. 3
Fig. 3 Comparison of LSK cells and P-selectin in peripheral blood between C57BL/6J wild-type and P-selectin-deficiency mice.The experimental outline used to monitor LSK cells and P-selectin in peripheral blood is illustrated (A).C57BL/6J wild-type (WT) mice (n = 7, five experiments with seven mice per group) and P-selectin-deficiency mice (Selp −/− ; n = 6, five experiments with six mice per group) were injected with G-CSF (250 μg/kg/day) for 4 consecutive days.P-selectin-deficiency mice (Selp −/− ; n = 6, five experiments with six mice per group) were injected with WT platelets (1 × 10 8 ) once a day, together with the last three doses of G-CSF.LSK cell numbers were detected using flow cytometry (FC) 1 week before and on day 4 after G-CSF treatment.The LSK cell numbers of G-CSF treated-WT mice were set as onefold.Each group's relative folds of LSK cell numbers are shown (B).The concentrations of P-selectin in either microparticles or soluble forms in the peripheral blood of each group were further characterized using immunoassays (C).Two groups were included in the study: Selp −/− mice injected with G-CSF (n = 4, two experiments with four mice per group) and Selp −/− mice injected with G-CSF and WT platelets (n = 4, two experiments with four mice per group).Data are reported as mean ± SD. **P < 0.01 compared with the microparticle groups within each group.## P < 0.01 compared with the indicated groups.

Fig. 4
Fig.4 Comparison of LSK cell numbers in the peripheral blood between mice treated with G-CSF, soluble P-selectin, and combined G-CSF and soluble P-selectin.The experimental outline for monitoring LSK cells in the peripheral blood is illustrated (A).C57BL/6J wild-type (WT) mice were injected with 4 consecutive days of G-CSF (250 μg/kg/day, n = 6, three experiments with six mice per group), combined G-CSF (250 μg/kg/day) and sP-sel (0.1 mg/kg, n = 4, one experiment with four mice per group), or three different doses of sP-sel (0.1 mg/kg, n = 6, two experiments with six mice per group; 0.5 mg/kg, n = 4, two experiments with four mice per group; 1.0 mg/kg, n = 6, two experiments with six mice per group).C57BL/6J mice injected with human IgG Fc fragment (1 mg/kg, n = 4, one experiment with four mice per group) served as a control group.The LSK cells in peripheral blood were analyzed and quantified using flow cytometry (FC) (B).Data are reported as mean ± SD. *P < 0.05 and **P < 0.01 compared with the before experiment groups (Before Exp.).## P < 0.01 compared with the indicated groups.

Fig. 5
Fig. 5 Comparison of P-selectin positive cell percentages in LSK populations between G-CSF-and soluble-P-selectin-treated groups.The experimental procedure for assessing the percentage of P-selectin positive (P-sel + ) cells in LSK groups is shown (A).C57BL/6J wild-type (WT) mice were injected with either 4 consecutive days of G-CSF (250 μg/kg/day, n = 4, one experiments with four mice per group) or two doses of sP-sel (1 mg/kg, n = 4, one experiments with four mice per group).The LSK cell counts in peripheral blood (B) and the percentages of P-sel + cells within LSK populations (C) were determined and quantified using flow cytometry (FC) 17 h after the final injection.Data are presented as mean ± SD. ** P < 0.01 compared with the indicated groups AbbreviationsG-CSFGranulocyte colony-stimulating factor HSCs Hematopoietic stem cells PSGL-1 P-selectin glycoprotein ligand-1 ELISA Enzyme-linked immunosorbent assay sP-sel Soluble P-selectin BM Bone marrow SDF-1 Stromal-derived factor-1 CXCR4 CXC chemokine receptor 4 IL Interleukin S1P Sphingosine-1-phosphate Selp −/− mice P-selectin-deficiency mice DPP4 Dipeptidyl peptidase 4 NPY Neuropeptide PBS Phosphate-buffered saline