Role of Oct4 in maintaining and regaining stem cell pluripotency

Pluripotency, a characteristic of cells in the inner cell mass of the mammalian preimplantation blastocyst as well as of embryonic stem cells, is defined as the ability of a cell to generate all of the cell types of an organism. A group of transcription factors is essential for the establishment and maintenance of the pluripotent state. Recent studies have demonstrated that differentiated somatic cells could be reverted to a pluripotent state by the overexpression of a set of transcription factors, further highlighting the significance of transcription factors in the control of pluripotency. Among these factors, a member of the POU transcription factor family, Oct4, is central to the machinery governing pluripotency. Oct4 is highly expressed in pluripotent cells and becomes silenced upon differentiation. Interestingly, the precise expression level of Oct4 determines the fate of embryonic stem cells. Therefore, to control the expression of Oct4 precisely, a variety of regulators function at multiple levels, including transcription, translation of mRNA and post-translational modification. Additionally, in cooperation with Sox2, Nanog and other members of the core transcriptional regulatory circuitry, Oct4 activates both protein-coding genes and noncoding RNAs necessary for pluripotency. Simultaneously, in association with transcriptional repressive complexes, Oct4 represses another set of targets involved in developmental processes. Importantly, Oct4 can re-establish pluripotency in somatic cells, and proper reprogramming of Oct4 expression is indispensable for deriving genuine induced pluripotent stem cell lines. In the past several years, genome-wide identification of Oct4 target genes and Oct4-centered protein interactomes has been reported, indicating that Oct4 exerts tight control over pluripotency regulator expression and protects embryonic stem cells in an undifferentiated state. Nevertheless, further investigation is required to fully elucidate the underlying molecular mechanisms through which Oct4 maintains and reinitiates pluripotency. Systemic and dynamic exploration of the protein complexes and target genes associated with Oct4 will help to elucidate the role of Oct4 more comprehensively.

domain and a C-terminal domain, which appears to be a cell type-specifi c transactivation domain. Oct4 belongs to the POU transcription factor family, in which the members control the expression of their target genes through binding an octameric sequence motif of an AGTCAAAT consensus sequence [8].
During mouse embryonic development, Oct4 is highly expressed in pluripotent embryonic cells, as well as cells of the germline, and its expression rapidly decreases upon diff erentiation. Analysis of upstream regulatory elements of the Oct4 genomic locus identifi ed a proximal enhancer and a distal enhancer, in addition to a TATA-less proximal promoter [9]. Interestingly, the proximal enhancer and the distal enhancer function at diff erent developmental stages: the proximal enhancer functions in the epiblast and epiblast stem cells, while the distal enhancer plays roles in the ICM, primordial germ cells and ESCs. Nordhoff and colleagues compared human, bovine and murine Oct4 upstream sequences and found four conserved regions (CR1 to CR4), showing possible involvement in Oct4 expression [10]. For more details on this topic, we refer readers to several excellent reviews [11,12].
Functionally, Oct4 is essential for early embryonic development, although it is not required for mouse somatic stem cell self-renewal [13]. In the absence of Oct4, embryos die at the time of implantation because of a lack of pluripotent ICM cells [14]. Oct4 is therefore considered a master regulator for the initiation and maintenance of pluripotent cells during embryonic development. Interestingly, the precise expression level of Oct4 is a critical determinant of ESC fates, and their pluripotent potential can be sustained only when the Oct4 expression level is maintained within a normal range [15][16][17]. Reducing Oct4 expression by one-half induces ESCs to diff erentiate into trophoblasts, while less than twofold overexpression of Oct4 triggers ESC diff erentiation into primitive endoderm and mesoderm [17]. In line with this, transforming growth factor betainduced transient Oct4 upregulation can promote cardiac mesoderm diff erentiation, supporting the role of the level of Oct4 in regulating pluripotency and diff erentiation [18]. Moreover, the recent discovery of an essential role of Oct4 in reprogramming somatic cells into pluripotent cells further highlights its irreplaceable function in establishing pluripotency.
In the present review, we fi rst summarize recent progress in understanding how the regulation of Oct4 expression is achieved at multiple levels, and then describe how Oct4 acts with its partners to regulate the expression of its target genes in maintaining pluripotency. Finally, we briefl y review the role of Oct4 in regaining pluripotency. Owing to limitations of space, we cannot cover all related studies and apologize to the authors whose contributions are not mentioned in this review.

Tight control of Oct4 expression at multiple levels
Achieving precise control of Oct4 expression is a key issue for the maintenance of as well as the re-establishment of pluripotency, and many players participate in this process at multiple levels ( Figure 1). First, a defi ned chromatin state is essential for Oct4 expression. In undiff erentiated ESCs, the Oct4 locus is hypomethylated and packaged with nucleosomes containing highly acetylated histone H3 (Lys 9, Lys 14) and dimethylated or trimethylated histone H3 (Lys 4) [19]. Th e locus, however, undergoes a series of epigenetic modifi cations, leading to the repression of Oct4 expression upon the induction of diff erentiation. In addition to deacetylation, G9amediated histone H3 methylation on Lys 9 recruits heterochromatin protein 1 to confer a compact chroma tin confi guration [19]. Subsequently, permanent inactiva tion of Oct4 is achieved when two de novo methyl transferases, Dnmt3a and Dnmt3b, function synergistically to methylate the promoter of Oct4 [20]. In addition, Cdk2ap1 was shown to promote Oct4 promoter methylation and to downregulate Oct4 expression during mouse ESC diff eren tiation through its direct interaction with the methyl DNA-binding protein Mbd3 [21]. Additionally, Paf1C, a component of the Paf1 complex, associates with RNA polymerase II and binds to the promoter of Oct4 to maintain a transcriptionally active chromatin structure [22]. Th e epigenetic regulation of the chromatin state therefore plays an important role in the control of Oct4 expression and the proper reprogramming of somatic cells to overcome repressive modifi cations. Indeed, inhibitors of G9a and DNA methyltransferase have been shown to promote the reprogramming effi ciency in a variety of cell types [23].
At the transcriptional level, direct binding of transacting regulators to the Oct4 locus also plays a critical role in the modulation of Oct4 expression. Several members of the orphan nuclear receptor family have been recently found to participate in the control of Oct4 expression, supporting an earlier report that the nuclear receptors PAR-1/COUP-TFII, EAR-3/COUP-TFI and RAR/RXR regulate the Oct4 promoter activity in mouse embryonal carcinoma cells [24]. Steroidogenic factor-1 and estrogen-related receptor b (Esrrb) were shown to directly activate the transcription of human OCT4 and mouse Oct4, respectively, to sustain pluripotency in ESCs [25,26]. Another orphan nuclear receptor, LRH-1 (also known as Nr5a2), was shown to bind the steroidogenic factor-1 responsive element in the proximal promoter and the proximal enhancer of Oct4 to maintain Oct4 expression at the epiblast stage of embryonic development [27]. Additionally, germ cell nuclear factor represses Oct4 expression by specifi cally binding with the proximal promoter and is necessary for confi ning Oct4 expression to the germline [28]. Interestingly, the orphan nuclear receptor Tr2 alternates between being an activator and a repressor of Oct4 expression depending on its phosphory lation, sumoylation and associated coregulators [29,30]. Furthermore, pluripotency factors form a regulatory circuitry consisting of autoregulatory and feedforward loops [31,32]. For instance, the distal enhancer of Oct4 has a composite Oct-Sox element, and Oct4 and Sox2 bind to this element to synergistically activate the expression of Oct4 [33]. Sall4, a spalt family member, also modulates ESC pluripotency through transcriptional control of Oct4 [34,35]. Th e coordinated action of these key factors is therefore fundamental for the maintenance of pluripotency.
miRNAs play important roles in the post-trans criptional regulation of Oct4 expression. Xu and colleagues reported that miR-145 directly repressed the 3' untranslated region of OCT4 mRNA during human ESC diff erentiation. Interestingly, the promoter of miR-145 was also observed to be bound and inhibited by OCT4 in human ESCs, uncovering a double-negative feedback loop for OCT4 and miRNA expression [36]. Although miRNAs usually target the 3' untranslated regions of mRNAs, miR-296, miR-470 and miR-134 were found to be upregulated in diff erentiated mouse ESCs and to target the coding sequences of Oct4, Nanog and Sox2 in various combinations [37]. In addition to these negative regulators of Oct4 during ESC diff erentiation, Qiu and colleagues recently found that Lin28 -which is a repressor of miRNA processing -bound OCT4 mRNA directly within its coding region and recruited RNA helicase A to promote the translation of OCT4 in human ESCs [38]. Th ere may still be many other regulators functioning at the post-transcriptional level that remain to be found.
Th e stability and activity of Oct4 proteins are subject to modifi cations at the post-translational level. An early study suggested that the diff erential phosphorylation of Oct4 might aff ect the transactivation ability of the Oct4 carboxyl-terminal transactivation domain [39]. A recent study reported that human OCT4 can be modifi ed by the monosaccharide O-linked β-N-acetylglucosamine (O-GlcNAc), which regulates the activity of a wide variety of cellular proteins [40]. Notably, the enzyme catalyz ing this post-translational reaction, Ogt, was found in Oct4-associated protein complexes in two recent independent studies [41,42]. Whether this modification regulates the OCT4 activity in ESCs, however, remains unclear. Moreover, ubiquitination is a common modifi cation of proteins that decreases the stability of modifi ed proteins. Our laboratory fi rst identifi ed a mouse HECT-type E3 ubiquitin ligase of Oct4 known as Wwp2 [43]. Subsequent research indicated that Wwp2 plays an important role in Oct4 ubiquitination and degradation during the diff erentiation of embryonic carcinoma cells, The chromatin modifi er Paf1C increases Oct4 expression by relaxing the compact chromatin, while G9a adds a repressive mark and recruits heterochromatin protein 1 (HP1), leading to a compact chromatin confi guration. Subsequently, Dnmt3a/b methylates the Oct4 promoter to permanently inactivate Oct4 expression. This process is enhanced by Cdk2ap1 interacting with Mbd3. At the transcription level, two sets of transcription factors and nuclear receptors function antagonistically: one set (including Esrrb, LRH-1, Oct4, Sox2 and Sall4) activates Oct4 expression, while the other set (including germ cell nuclear factor (GCNF) and Tr2) represses its expression. After transcription, Lin28 recruits RNA helicase A to promote the translation of Oct4 mRNA, while miRNAs (including miR-134 and miR-470) cause its decay. In addition, several posttranslational modifi cations (such as ubiquitination (Ub), sumoylation and phosphorylation (P)) are added to the Oct4 protein after it is translated and infl uence the activity and stability of Oct4 proteins. SUMO, small ubiquitin-related modifi er.
although it does not appear to aff ect Oct4 protein levels in undiff erentiated mouse embryonic carcinoma cells and ESCs [44]. Interestingly, its human counterpart, WWP2, can ubiquitinate endogenous OCT4 proteins in human ESCs and promote its degradation through the 26S proteasome [45]. Currently, there exists no defi nitive answer to the question of why this enzyme degrades OCT4 proteins in undiff erentiated human ESCs but not in undiff erentiated mouse ESCs. One possible explanation is that human ESCs represent a more developed stage than mouse ESCs [46]. In addition to ubiquitination, our group and another group found that Oct4 was a target of small ubiquitin-related modifi er-1 modifi cation and that the sumoylation resulted in increased stability, DNA binding and transactivation of Oct4 [47,48]. Ubiquitination and sumoylation appear to jointly control Oct4 proteins at an appropriate level in ESCs. We hypothesize that other types of modifi cations of Oct4 proteins may exist to regulate its protein level and function.
Th e Oct4 expression level is modulated by multiple factors and mechanisms. Continued eff orts are required to elucidate the sophisticated regulatory network associated with the precise control of Oct4 expression, particu larly to understand how Oct4 expression is reactivated during reprogramming.

Transcriptional targets and protein complexes associated with the function of Oct4
Oct4 lies at the center of the mechanisms that maintain the self-renewal and pluripotency of ESCs. Oct4 mainly functions through the activation of pluripotencyassociated and self-renewal-associated genes, while simul taneously repressing genes that promote diff erentiation, in coordination with other pluripotency factors and coregulators. Genome-wide mapping of the binding sites of Oct4 and other ESC factors has revealed that Oct4 clusters with diff erent but overlapping sets of transcriptional factors or coregulators at distinct genomic locations, suggesting that the function of Oct4 in the regulation of gene expression may be modulated by various associated partners. Among the partners of Oct4, Sox2 is the best characterized. Th ey are considered as a versatile pair of master regulators orchestrating selfrenewal and pluripotency. Th e regulatory elements of a large number of Oct4 target genes contain a composite Oct-Sox element separated by several nucleotides. Oct4 and Sox2 bind the element simultaneously and synergistically activate the expression of these genes, including Oct4 [33], Sox2 [33], Nanog [49], Fgf4 [50], Utf1 [51] and Zfp206 [52], all of which play important roles in maintaining pluripotency. Subsequent studies have shown that certain Oct4/Sox2-targeted transcription factors are themselves often components of the Oct4 interactome. For instance, Oct4, Sox2 and Nanog have been con sidered to act as a core transcriptional regulatory circuitry in pluripotent stem cells, as they co-occupy a large number of their target genes [31,53,54]. Nanog could be an important target for Oct4 in maintaining pluripotency; it is required for repressing the diff erentiation of pluripotent epiblast cells toward extraembryonic lineages [49,55,56], and its overexpression can maintain ESCs in an undiff erentiated state in the absence of leukemia inhibitory factor. Additionally, Zfp206 was recently demonstrated to physically interact with Oct4/ Sox2, being a key component of Oct4/Sox2 complexes [57]. Th e Oct4 transcriptional regulatory circuitry thus ensures tight control of pluripotency regulator expression and maintains ESCs in an undiff erentiated state.
Several well-known Oct4 target genes -including Fgf4, Utf1, Osteopontin, Rex1, Hand1 and α and β human chorionic gonadotropin (hCG) -were identifi ed through earlier single-gene experiments using promoter-reporter or enhancer-reporter activity assays (reviewed in [11,12]). To identify Oct4 targets at a global level, high-throughput approaches such as gene-expression profi ling in Oct4manipulated mouse ESCs, genome-wide chromatin immuno precipitation assays or a combination of both have been utilized in the past several years [32,58,59]. Th ese investigations identifi ed hundreds and thousands of putative Oct4 targets. Notably, Oct4 appears to function predominantly as an activator of gene expression, although it also suppresses transcription [59]. Interestingly, Matoba and colleagues discovered a group of targets that exhibit both activation and suppression depending on the Oct4 expression level [58]. Rex1 is an example of this unique type of regulation, as at both low and high levels Oct4 represses Rex1 expression but at intermediate levels it activates Rex1 expression. Th is fi nding may be related to mechanisms underlying the requirement to keep the Oct4 level within a narrow range for the main tenance of ESCs in a self-renewal and pluripotent state [17]. In addition to mouse ESCs, chromatin immuno precipitation-on-chip experiments have also been performed in human ESCs to reveal DNAprotein binding events involving OCT4, SOX2 and NANOG. Th e target genes identifi ed frequently encode transcription factors, many of which are developmentally important homeodomain proteins [31]. Moreover, Chavez and colleagues reported the identifi cation of a core OCT4 regulatory network in human ESCs consisting of 33 target genes by carrying out an integrated analysis of high-throughput data [60]. More recently, two groups reported the genome-wide identifi cation of binding sites of diff erent sets of ESC factors [61,62]. Th eir results show that genes in which the promoters are occupied by multiple factors, such as Oct4, Sox2, Nanog and Klf4, are generally active in ESCs; in contrast, genes that bind fewer or single factors tend to be inactive or repressed [62]. ESC-specifi c gene expression and the state of pluripotency are therefore orchestrated by the coordinated eff orts of many key transcriptional factors and coregulators.
In addition to activating pluripotency factors, Oct4 also prevents the diff erentiation of pluripotent cells by acting as a repressor of lineage-specifi c transcription factors. During the fi rst cell fate-deciding event in mammalian development -the segregation of the ICM and the trophectoderm -Oct4 is required for establishing the pluripotency of ICM cells. Oct4 forms a repressive complex with and inhibits the transcription of Cdx2, a trans cription factor essential for trophectoderm specification [63]. In the absence of Oct4, the embryo develops to the blastocyst stage and appears to be morphologically normal. Th e cells in the ICM, however, diff erentiate into trophoblast cells without pluripotent embryonic cells [14]. Moreover, Oct4 recruits a histone H3 (Lys 9) methyl transferase, Eset, to silence the expression of trophoblast-associated genes, thus partnering with an epigenetic regulator to restrict extraembryonic trophoblast lineage potential in ESCs [64]. At the late blastocyst stage, Oct4, Sox2 and Nanog are required for the development of pluripotent epiblast cells and the restriction of the formation of primitive endoderm. A recent study by our group demonstrated that Oct4, together with Sox2 and Nanog, directly inhibits the expression of Stk40, an inducer of extraembryonic endoderm in mouse ESCs. In agreement with the notion that Oct4 functions to prevent ICM cells from diff erentiating into primitive endoderm, we found that ESCs depleted of Oct4 by specifi c interfering RNA were mainly incorporated into the extra embryonic endodermal lineages in chimeric embryos when injected into preimplantation blastocysts [16]. Furthermore, it was reported that Oct4 interacts with the DNA-binding domain of FoxD3 and represses its activation of FoxA1 and FoxA2, two critical regulators for the development of the endodermal foregut [65]. Th e subsequent identifi cation of Oct4-interacting proteins by affi nity purifi cation and mass spectrometry revealed that Oct4 associated with unique transcriptional repression complexes containing Hdac1/2 and Mta1/2 in mouse ESCs [66,67], providing signifi cant insight into the mechanism of the repression of developmental genes by Oct4.
Recently, using improved affi nity tagging approaches, van den Berg and colleagues and Pardo and colleagues identifi ed 50 and 92 putative Oct4-associated proteins, respectively, in mouse ESCs [41,42]. Th ese two studies not only identifi ed more detailed Oct4-centered interactome networks than previous analyses, but also detected interactions between Oct4 and signaling pathways. For example, van den Berg and colleagues found that Oct4 associated with Rbpi, the nuclear eff ector of the Notch signaling pathway -implying a connection between Oct4 and the Notch-regulated gene expression. In addition, both interactomes contain chromatinmodifying complexes, such as NuRD and SWI/SNF, repre senting links between epigenetic modify ing complexes and pluripotency transcriptional factors. Despite substantial progress in understanding Oct4-centered protein interactomes in ESCs, there are signifi cant discrepancies between these two studies [68]. Further investigation and detailed comparison are required to clarify the basis of the diff erences.
In addition to protein coding genes, noncoding RNA targets of Oct4 have been found. For example, in concert with Sox2, Oct4 positively regulates miR-302, a cluster of eight miRNAs expressed specifi cally in ESCs. One member of this cluster, miR-302a, inhibits the translation of cyclin D 1 (an important G 1 regulator) to maintain an ESC-specifi c cell cycle profi le [69]. Moreover, a long noncoding RNA, AK028326, which was recently shown to be directly activated by Oct4, was observed to function as a coactivator of Oct4 in a regulatory feedback loop [70]. Functionally, the overexpression or knockdown of AK028326 changed the expression levels of pluripotency factors, as well as lineage-specifi c factors, indicating its functional role in determining the cell fate of ESCs. In addition, Oct4 also functions during X-chromosome reprogramming. It can directly binds Tsix and Xite (two regulatory noncoding RNA genes of the X-inactivation center) and also interacts with Ctcf (the X-chromosome inactivation trans-factor) to regulate X-chromosome pairing and counting [71]. Th ese studies expanded our knowledge of the Oct4-centered transcriptional regulatory network in pluri potent cells, and their results have elucidated new mechanisms associated with establishing and maintain ing pluripotency.
Taken together, Oct4 interacts with other pluripotency factors -such as Nanog, Sox2, Sall4, Klf5, Zfp143, Zfp206, Esrrb, Dax1 and Tcfcp2l1 -to form autoregulatory and cross-regulatory loops and to maintain a pluripotent state. Interestingly, some of these factors, including Esrrb, Tcfcp2l1 and Dax1, depend on Oct4 for the effi cient targeting of several of their shared sites [41], emphasizing the importance of coordination among pluripotency factors and the critical role of Oct4 in the maintenance of a pluripotent state. Additionally, Oct4 may recruit transcriptional repressive complexes, such as NuRD and PRC1, to the genomic sites of lineage-specifi c factors and prevent diff erentiation of pluripotent cells (Figure 2).

Indispensable role of Oct4 in reprogramming
Th e achi evement of reprogramming by four defi ned transcription factors drew tremendous interest in the scientifi c fi eld. Th e earliest reprogramming was achieved using the Yamanaka factors (Oct4, Sox2, Klf4 and c-Myc) [2] or the Th omson factors (Oct4, Sox2, Nanog and Lin28) [3], both of which include Oct4. It was later found that some cell types express one or several reprogramming factors endogenously and require fewer factors for reprogramming. Other factors and some family members of reprogramming factors, as well as certain chemicals, promote the reprogramming process (reviewed in [23]). Noticeably, Oct4 was required in all except two studies to derive induced pluripotent stem (iPS) cell lines: one study found that BIX-01294, an inhibitor of the G9a histone methyltransferase, could reprogram mouse neural progenitor cells in concert with Sox2, Klf4 and c-Myc [72]; the other study found that Nr5a2 and its close family member Nr5a1 could replace Oct4 in the reprogramming of mouse embryonic fi broblasts [73]. Th e effi ciency of reprogramming was extremely low in both reports, however, and these substitutes might function through modulating Oct4 and Nanog expression. In addition, Fbx15 (a marker gene of undiff erentiated ESCs that is dispensable for pluripotency in ESCs) was utilized as a reporter of reprogramming in the fi rst generation of iPS cells. Th e derived iPS cell lines, however, were dissimilar to ESCs in several aspects and could not generate chimeras [2]. Further characterization of these lines found that the promoter of the endogenous Oct4 gene was still highly methylated. In later research, the state of the hypomethylated Oct4 promoter was used as an indicator of complete reprogramming, and the resultant iPS cell lines could not be distinguished from ESCs, further emphasizing the importance of Oct4 in regaining pluripotency [74]. Moreover, the derivation of iPS cell lines requires viral transfection, hampering their clinical application; one way of avoiding this problem is to use fewer reprogramming factors. Scholer's group successfully reprogrammed both mouse and human neural progenitors using only Oct4 [75,76], again highlighting the power of Oct4 in re-establishing pluripotency.
Having demonstrated the essential and critical role of Oct4 in regaining pluripotency, scientists have engaged Depending on its associated partners, Oct4 can either activate or repress its target genes. When associated with factors such as Sox2, Nanog and Sall4 at regulatory elements, Oct4 usually exerts stimulatory eff ects on gene expression. Additionally, Oct4 can repress developmental genes through interacting with repressors (for example, NuRD and PRC1). Through regulating various targets, Oct4 has versatile eff ects on pluripotency: it maintains self-renewal and pluripotency by activating its own expression and that of factors such as Sox2 and Nanog; through modulating the expression or function of development-associated genes -such as Stk40, Cdx2, Eset and FoxD3 -Oct4 prevents the diff erentiation of pluripotent stem cells; it can also infl uence other aspects of embryonic stem cells (ESCs), including cell cycle and X-chromosome inactivation, through various eff ectors (for example, miR-302a, Ctcf ). ExEn, extraembryonic endoderm. in tremendous eff orts to understand the underlying mechanisms of reprogramming, especially the roles of Oct4 during the process of reprogramming. Preliminary results showed that Oct4 plays an important role in activating ESC-specifi c genes by cooperating with Sox2 and Klf4, and most of these genes are silenced in partially reprogrammed cells [77]. Moreover, Oct4 was reported to suppress the transcription of the epithelial mesenchymal transition mediator Snail to facilitate reprogramming [78]. Interestingly, a new strategy for Oct4 functions was recently suggested -that is, Oct4 specifi cally interacts with nuclear β-catenin and facilitates its proteasomal degradation, thus maintaining the undiff erentiated state of ESCs [79]. Th e important conclusions from these studies are that Oct4 does not function alone and that a delicate balance among pluripotency factors defi nes the state of pluripotency in ESCs and embryonic cells. Similarly, regaining this balance is the key to successful reprogramming, which might, at least partially, explain why reprogramming effi ciency is very low, even when four factors are effi ciently transduced into somatic cells.

Conclusion and perspective
Since Oct4 was fi rst identifi ed in mice as an embryonic stem cell-specifi c and germline-specifi c transcription factor in 1990, studies have highlighted its importance in governing pluripotency. Th e complex regulation of Oct4 expression further refl ects the necessity to control its expression level precisely. Th e interest in Oct4 increases primarily due to the indispensable role of Oct4 in the generation of iPS cells. Nevertheless, a number of important issues remain to be addressed.
Th e study of gene expression profi ling indicates that Oct4 primarily acts as an activator of gene expression [59]. In Oct4 protein interactomes, however, many Oct4associated partners have been identifi ed as trans criptional repressors [41,42,66,67]. Notably, the coactivator p300 was found to be recruited to the cluster of Oct4, Sox2 and Nanog using a chromatin immunoprecipitationseq approach [61], implying that p300 might play a role in Oct4-mediated transcription activation. Strangely, none of the recently identifi ed Oct4-centered protein interaction networks contain p300.
How Oct4 connects to the basal transcriptional machinery also remains unclear. Van den Berg and colleagues showed the association of Esrrb with components of the basal transcriptional machinery, including the mediator complex, RNA polymerase II subunits and TATA box binding protein plus transcription-associated factors. Th e connection between Oct4 and the basal transcriptional machinery could thus be established through Esrrb, as the association of Oct4 with Esrrb has been documented [56]. Furthermore, it remains unclear how diff erent dosages of Oct4 act to determine ESC fates. One possibility is that Oct4 associates with distinct partners at diff erent dosages, provided that Oct4 displays diff erent affi nities toward its partners at diff erent expression levels. Achieving a comprehensive understanding of how diff erent biological processes, such as transcriptional, posttranscrip tional and epigenetic regulation, work together to maintain Oct4 at an appropriate level will also help to solve this puzzle. Another interesting and important question is why Oct4 expression is so critical in generating fully reprogrammed cells from somatic cells.
Clearly, the investigation of how Oct4 exerts its functions through regulating various targets is in its early stages, and this should be the focus of future research. In addition, employing an experimental design to study Oct4-associated proteins or target genes at a single-cell level in a dynamic manner will allow us to obtain a clearer picture of how Oct4 maintains and re-establishes pluripotency. We believe that these types of investigations and other related studies will fulfi ll the great promise of pluripotent stem cells in the near future.

Competing interests
The authors declare that they have no competing interests.