Figure 2

Effect of Y-27632 on the culturing of keratinocytes. (A) The morphology of keratinocytes from the three groups at the indicated time points (P0, P5, and P10). (B) The growth curves of keratinocytes with various treatments. (C) Beta-galactosidase staining of keratinocytes from the three groups at the indicated time points (P0, P5, and P10). (D) The average number of cells staining positive for β-galactosidase was divided by the average total number of cells (nuclei counts) and multiplied by 100 to obtain the percentage. Data are presented as the mean ± standard error of the mean. n = 3; scale bar: 50 μm. **P<0.01 keratinocytes without Y-27632 versus keratinocytes with Y-27632, ##P<0.01 keratinocytes without Y-27632 versus Y-27632 added to keratinocytes at passage 5. DKSFM, defined keratinocyte serum-free medium; DKSFM + Y, defined keratinocyte serum-free medium supplemented with Y-27632 at passage 0; DKSFM + YP5, defined keratinocyte serum-free medium supplemented with Y-27632 at passage 5; Y, Y-27632; YP5, Y-27632 added to keratinocytes at passage 5.