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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats

Figure 1

Cryopreserved umbilical cord blood mononuclear cells (UCB-MNCs) differentiated into endothelial progenitor cells (EPCs) under endothelial cell-specific culture conditions. Culture of MNCs resulted in the emergence of colonies (A, B, and C). Cells double-labeled for fluorescein isothiocyanate (FITC)-lectin binding (green, D) and DiI-labeled acetylated low-density lipoprotein (DiI-LDL) uptake (red, E) were identified as EPCs (yellow, F). Tube formation assay (G and H, red arrow) indicated endothelial function. Transmission electron microscope image of cultured cells indicates the existence of Weibel-Palade bodies, a typical superstructure of endothelial cells (I, yellow arrow).

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