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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats

Figure 4

Increased protein expression of vascular endothelial growth factor (VEGF) and stromal cell-derived growth factor 1 (SDF-1) in EPCs compared to mature endothelial cells and MNCs. (A) A representative western blot of three independent experiments. GAPDH serves as the loading control. (B) Protein expression quantified by densitometry analysis. The ratio for VEGF or SDF-1 loading is shown. (C and D) HUVECs, EPCs, and MNCs were incubated with equal amounts of fresh medium for 24 hours. The medium was collected, 20× concentrated, and measured by ELISA, n ≥ 6, ** P <0.001 versus HUVECs and MNCs. EPCs, endothelial progenitor cells; HUVECs, human umbilical venous endothelial cells; MNCs, mononuclear cells.

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