Figure 9

Hepatocyte growth factor (HGF) was secreted in mesenchymal stem cell-endothelial cell (MSC-EC) interaction conditions and restored remodelling of vascular endothelial (VE)-cadherin. The results indicated that human pulmonary microvascular endothelial cells cultured under normal conditions had VE-cadherin, the main component of adherens junctions, forming intercellular junctions. Lipopolysaccharide (LPS) induced dispersion of VE-cadherin. After 7 days of direct or indirect co-cultured with human MSCs, VE-cadherin was partially restored. However, neutralising HGF from conditioned media with anti-HGF antibody (Ab) caused VE-cadherin to be disrupted again. DAPI, 4,6-diamidino-2-phenylindole.