Figure 4From: Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads, with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing Senescence and karyotype analysis. SA-β-gal staining of 2D and 3D cultured hWJ-MSC; blue color represents senescent cells. Scale bar = 50 μm. (A) Data were not statistically significantly different between the two groups; cells were counted from three different fields and the average determined. (B) Representative karyotypes of cells cultured under 2D and 3D conditions at passage 6. (C). hWJ-MSC, human Wharton’s jelly-derived mesenchymal stem cells; SA-β-gal, senescence-associated β-galactosidase; 2D, plate cultures; 3D, spinning bottle cultures.Back to article page