Figure 8
Efficacy of hWJ-MSC in mouse skin wound closure. Representative images of the mouse excisional wound splinting model after transplantations of 2D culture-derived cells, 3D culture-derived cells, phosphate-buffered saline (PBS), and control vehicle medium (sham) at days 3, 7, 14, and 21 (A). Wound measurement in each group of mice; n = 6 per group. *P <0.05, for treatment with 2D cultured cells versus PBS, and with 3D cultured cells versus PBS, at day 14. (B). Percentage of histological wound healing; n = 6 per group, for treatment with 2D cultured cells versus PBS; *P <0.05; or for 3D cultured cells versus PBS; *P <0.05; or for 2D cultured cells versus 3D cultured cells; P >0.05, at day 14 (C). Wound histology after hematoxylin and eosin staining. Tissue sections obtained from the wound area at day 14 after cell injection were stained with antibodies against cytokeratin 14 (red) and CD31 (red). Fluorescence microscopy showed that cytokeratin (red) was expressed in the newly formed epidermis and that CD31 (red) was present in the newly formed blood vessels (D). The thickness of the new epidermis was measured using Image J. There was no significant difference between groups at days 7 and 21 after transplantation. The thickness of the newly formed epidermis in animals treated with 2D and 3D cultured cells were both greater than that seen in the PBS- and sham-treated groups (E). A histogram representing the number of newly formed blood vessels in 1 mm2 by day 14 after transplantation. 2D versus 3D cultured cells, P >0.01; 2D cultured cells versus PBS, P <0.05, 3D cultured cells versus PBS, P <0.05; 2D cultured cells versus sham, P >0.05, 3D cultured cells versus sham, P >0.05 (F). hWJ-MSC, human Wharton’s jelly-derived mesenchymal stem cells; 2D, plate cultures; 3D, spinning bottle cultures.