Figure 3

Mesenchymal stromal cell (MSC) conditioned medium (CM) alters gene expression levels in dermal fibroblasts (n = 3). (A) Schematic illustration of NBL-6 treatments for RNA isolation. Fold change of mRNA levels, as detected by quantitative reverse transcription-polymerase chain reaction, in (B) NBL-6 cells cultured with MSC-derived CM as compared with control CM or (C) NBL-6 cells cultured with CM from preconditioned MSCs as compared with CM from non-preconditioned MSCs. Left panel shows genes involved in reduction of hypergranulation or enhancement of wound healing or both, and right panel shows genes that stimulate hypergranulation or delay wound healing or both. *P <0.05; **P <0.01. ANXA2, annexin A2; CCL2, CC chemokine 2; CoCl₂, cobalt chloride; COLI, collagen type 1A1; COLIII, collagen type 3; CTSK, cathepsin K; CxCL10, CXC chemokine 10; IFNγ, interferon-gamma; IL-8, interleukin-8; MMP1, metallopeptidase 1; MMP2, metallopeptidase 2; NBL-6, horse dermal fibroblast cell; PAI1, plasminogen activator inhibitor-1; PLAT, tissue plasminogen activator; PLAU, plasminogen activator urokinase; SDC2, syndecan 2; SDC4, syndecan 4; TIMP1, metallopeptidase inhibitor 1; TNFα, tumor necrosis factor-alpha.