Figure 1
From: Acyclic retinoid induces differentiation and apoptosis of murine hepatic stem cells
Hepatic stem cell isolation and dose-dependent response to acyclic retinoid. (A) Hepatic stem cells (HpSCs) were sorted by flow cytometry using the gate c-Kit−CD29+CD49f+/lowCD45−Ter-119−. (B) The expression of Rarα, Rarβ, Rxrα, and Rxrβ in adult liver (AL; n = 3), fetal liver (FL; n = 3), fetal liver cell (FLC; n = 3), and HpSCs (n = 3). (C) Acyclic retinoid (ACR) inhibited the growth of HpSCs with different concentrations (0, 0.5, 1.0, 2.0, 4.0, and 8.0 mg/L). Scale bar = 200 μm. (D) Relative numbers of HpSCs and numbers of HpSC colonies in different concentrations of ACR after 7 days of culture (n = 3). Data are shown as means ± SD. Mann–Whitney U-test. *P < 0.05; **P < 0.01.