Figure 2

Transcriptional regulators in the definitive hematopoietic development program. Lim-domain-binding 1 (Ldb1) stabilizes a complex of transcription factors involving Lim-motif 2 (Lmo2), stem cell leukemia (Scl), and GATA box binding protein 2 (GATA2) [29]. During hematopoietic differentiation, this complex induces the expression of runt related protein 1 (Runx1), which is required for definitive hematopoiesis, as reported previously [27]. Surface marker analysis revealed that generation of CD41⁺ cells is impaired in the absence of Ldb1.