Figure 13

Effect of mesenchymal stromal cell-conditioned medium (MSC-CoM) and fibroblast-conditioned medium (FB-CoM) on metabolic activity and gene expression in hypoxia. (A) MSC-CoM and FB-CoM effects on the activity of cytosolic and mitochondrial oxidoreductases determined by MTS assay, and (B) MSC-CoM and FB-CoM effects on ATP content in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs). Data are normalized for respective average of hypoxia and glucose/serum deprivation (GSD) control experiments without MSC-CoM. *P ≤ 0.05, versus hypoxia/GSD controls. (C) Effect of hypoxia on expression of stress response genes in iPS-CMs incubated in standard or MSC-CoM in hypoxia/GSD. Data are shown as fold-change relative to the expression in normoxia/full medium (=1). *P ≤ 0.05, versus normoxia/full medium; ^# P ≤ 0.05, control versus MSC-CoM. **P ≤ 0.05, overall by analysis of variance.