Figure 1

Experimental procedures. Experiment 1: Effects of implantation of DFAT cells on mAb 1-22-3-induced nephritis. In Wistar rats weighing 250 g, the right kidney was nephrectomized (Neflec). Rats were injected with 0.5 mg mAb 1-22-3 through the tail vein seven days after nephrectomy. Thirty-five days after nephrectomy, 1.0 mL saline or 10⁶ DFAT cells were injected through the renal artery or tail vein. Sixty-three days after nephrectomy, all rats were killed and the left kidney was removed. Experiment 2: Effects of implantation of DFAT cells on adriamycin-induced nephropathy. In all male Wistar rats, the right kidney was nephrectomized. Seven days after nephrectomy, rats were injected with 4 mg/kg body weight adriamycin through the tail vein and 10⁶ DFAT cells were injected through the renal artery or through the tail vein. Thirty-five days after the nephrectomy all rats were killed and the left kidney was removed. Experiment 3: DFAT cells (2 x 10⁵ cells) from Wistar rats were transfected with 20 nM rat TSG-6 siRNA or 20 nM control siRNA in siRNA Transfection Medium. In male Wistar rats weighing 250 g, the right kidney was nephrectomized. Rats were injected with 0.5 mg mAb 1-22-3 through the tail vein and 10⁶ DFAT cells transfected with TSG-6 siRNA or control siRNA were injected through the tail vein. Twenty-one days after the nephrectomy, all rats were killed and the left kidney was removed. DFAT, dedifferentiated fat; mAb, monoclonal antibody; TSG, TNF-stimulated gene.