Figure 4
Effects of implantation of DFAT cells on degeneration of renal cortex in mAb 1-22-3-induced glomerulonephritis. Wistar rats were nephrectomized and injected without mAb 1-22-3 (Control) or with 0.5 mg mAb 1-22-3 through the tail vein seven days after the nephrectomy. Thirty-five days after the nephrectomy, saline (Saline) or 106 DFAT cells were injected through the renal artery (DFAT ia) or tail vein (DFAT iv). Sixty-three days after the nephrectomy, the left kidney was removed. A) The paraffin sections of the removed renal cortex were stained with hematoxylin and eosin (H & E). Renal cortical thickness was measured under high magnification (x400). B) Glomerular injury score (GIS). C) Tubulointerstitial injury score (TIS). Data are the mean ± SEM (n = 6). *P <0.05 and **P <0.01 in the indicated columns. Bar = 50 μm. DFAT, dedifferentiated fat; mAb, monoclonal antibody; SEM, standard error of the mean.