Figure 3

Raman spectrum of different osteosarcoma cells. (A). Raman spectra (averaged over different parts of the cell samples) showing the expression level of hydroxyapatite (HA) in different cells. (B) Raman spectra revealing significant differences in the peak intensity at the symmetric P–O stretch band (around 960 cm⁻¹) of HA in different grades of osteosarcoma cells (SaOS2, 143B, and MG63). HA was highly expressed in MG63 cells, but not in human mesenchymal stem cells (hMSC) and SaOS2 and 143B cells. *P <0.05, **P <0.01. (C) Raman spectrum showing the symmetric P–O stretch band (around 960 cm⁻¹) of HA. hFOB, human fetal osteoblast.