Fig. 4
Systemic human MSC transplantation recovers IL-17-accelerated recipient BMMSC-mediated osteoclastogenesis in the bone marrow of MRL/lpr mice. a A schema of osteoclastic induction. Mouse bone marrow cells (BMCs) were co-cultured with wild-type mouse-derived calvarial cells (Calvaria-WT) or recipient bone marrow mesenchymal stem cells (BMMSCs) under stimulation with 1α, 25-(OH)2 vitamin D3 (VD3) and prostaglandin E2 (PGE2). Calvaria-WT and recipient BMMSCs were pretreated with or without recipient BMC-derived conditioned medium (CM) or interleukin-17 (IL17) in the presence of anti-mouse IL-17 antibody (Anti-IL-17 Ab) or control antibody for anti-mouse IL-17 antibody (Cont Ab). b–g Osteoclast induction assay by tartrate-resistant acid phosphate (TRAP) staining. n = 5 for all groups. Values are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.005. b Co-culture of Calvaria-WT and wild-type mice-derived bone marrow cells (BMC-WT). # P < 0.05, versus CM-MRL/lpr pretreatment in the presence of Cont Ab; ††P < 0.01, versus CM-MRL/lpr pretreatment in the presence of Anti-IL-17 Ab. c, d Co-culture of Calvaria-WT and recipient BMCs. ¶ P < 0.05, ¶¶ P < 0.01, ¶¶¶ P < 0.005, versus BMC-MRL/lpr and Calvaria-WT co-culture. e Co-culture of BMC-MRL/lpr and MSC-MRL/lpr. f, g Co-culture of BMC-MRL/lpr and recipient BMMSCs. ## P < 0.01, ### P < 0.005, versus co-culture of BMC-MRL/lpr and Cont Ab-pretreated MSC-MRL/lpr . f ††P < 0.01, †††P < 0.005 versus co-culture of BMC-MRL/lpr and CM-MRL/lpr-pretreated MSC-MRL/lpr. g ¶ P<0.05, versus co-culture of BMC-MRL/lpr and IL-17- and Cont Ab-pretreated MSC-MRL/lpr co-culture pretreated with IL-17; ∆ P < 0.05, ∆∆ P < 0.01, versus co-culture of BMC-MRL/lpr and IL-17- and Anti-IL-17 Ab-pretreated MSC-MRL/lpr. MNC multinucleated cell; MSC mesenchymal stem cell; SHED stem cells from exfoliated deciduous teeth