Fig. 4

The impact of miR 302/367 overexpression and Mbd3 depletion on induced pluripotent stem cell (iPSC) colony-forming efficiencies. (a) Exemplary alkaline phosphatase stainings of iPSCs generated from foreskin neonatal fibroblasts and urinary epithelial cells. Plates labelled as control show cells reprogrammed with episomes carrying OCT3/4, SOX2, KLF4, L-MYC, LIN28, and p53 dominant negative mutant. Cells labelled as + miR 302/367 were additionally transfected with episomal construct comprising mCherry-miR 302/367 cassette driven by cytomegalovirus promoter, whereas + Mbd3 shRNA indicates the cells additionally transfected with episomal vector carrying shRNA against Mbd3 mRNA under control of U6 promoter. (b) Summary of the reprogramming experiments with use of vectors carrying miR 302/367 and Mbd3 shRNA constructs. Graphed data show results of triplicate experiments presented as a mean ± standard error of the mean. Asterisks indicate statistically relevant difference between compared samples. Ctrl control, Mbd3 methyl-CpG-binding domain protein 3, shRNA short hairpin RNA