Fig. 1
Setup for culture of PCL filaments with MSC or MSC with SC. a,b Scanning electron microscopy (ESM) photomicrographs of PCL filaments showing in low (a) and high (b) magnification the microstructured grooves formed by melting extrusion at 60 °C. c Phase-contrast photomicrograph and c’ fluorescence photomicrograph in higher magnification of mesenchymal stem cells (MSC) in culture after three passages. In c’ MSC expressed enhanced green fluorescent protein (EGFP; green), and nuclei are labeled in blue (DAPI labeling). d Phase-contrast photomicrograph of Schwann cells (SC) derived from adult sciatic nerve cultured for 10 days. d’ Fluorescence photomicrograph of SC immunostained for S-100 (red) and nuclei labeled with DAPI (blue). The vast majority of cells are positive for S-100. e Photograph illustrating the culture system used, with a bundle of PCL filaments treated with plasma-O2/poly-D-lysine/laminin and incubated with MSC or MSC plus SC for 48 h. Scale bars: a = 50 μm; b = 10 μm; c,d = 400 μm; c’,d’ = 100 μm; e = 2 cm