Fig. 1
From: Development of a novel imaging system for cell therapy in the brain
Hipp-NSCs form neurospheres and generate neurons and glia in vitro. a A typical neurosphere formed by Hipp-NSCs growing in serum-free medium with EGF and FGF-2. b Immunofluorescence analysis of nestin expression (red) in Hipp-NSC neurospheres. Nuclei are stained blue with DAPI. c Phase-contrast photograph of Hipp-NSCs growing on poly-ornithine-coated plates in differentiation medium for 8 days. d Immunofluorescence analysis of Hipp-NSCs after 8 days of differentiation showing the expression of the neuronal marker βIII-tubulin (red) and of the glial marker GFAP (green). Nuclei are stained blue with DAPI. Scale bars = 50 μm. DAPI 4′,6-diamidino-2-phenylindole, EGF epidermal growth factor, FGF-2 fibroblast growth factor-2, GFAP glial fibrillary acidic protein, Hipp-NSC hippocampus-derived neural stem cell