Fig. 6
From: Development of a novel imaging system for cell therapy in the brain
In vivo SPECT imaging of NIS-Hipp-NSCs injected in the brain hippocampus. a SPECT signal recorded from NIS-Hipp-NSCs that were incubated with 99mTc in vitro before transplantation in the hippocampus (two sites/hippocampus). Transverse, coronal, and sagittal orientations of the brain are shown. b Correlation between the number of cells injected in the hippocampus and the signal detected by SPECT imaging. Correlation coefficient r = 0.9566; two-tailed P value = 0.0108. c Analysis of the brain shows grafted cells (identified by the expression of GFP) in the hippocampus. The expression of the NIS in the grafted cells was confirmed by immunofluorescence by using an antibody against hNIS (inset). GFP green fluorescent protein, Hipp-NSC hippocampus-derived neural stem cell, hNIS human sodium iodide symporter, NIS sodium iodide symporter, NIS-Hipp-NSC hippocampus-derived neural stem cell expressing the human sodium iodide symporter, SPECT single-photon emission tomography, 99m Tc technetium-99m