Fig. 6
From: Characterisation of neurons derived from a cortical human neural stem cell line CTX0E16
Phosphorylation of ERK1/2 kinase in differentiated CTX0E16 neurons following activation of neurotransmitter receptors. a, b Treatment with glutamate results in a time-dependent increase in phospho-ERK1/2 levels in differentiation day (DD) 28 CTX0E16 neurons. By 60 minutes, phospho-ERK1/2 levels are still raised as compared to control levels (a). Conversely, GABA application results in a transient increase in phospho-ERK1/2 levels; after 50 minutes, p-ERK1/2 levels have returned to baseline (b). c-e Treatment with dopamine (c) or 5-HT (d) results in activation of ERK1/2; CTX0E16 neurons respond to dopamine with a slower temporal profile as compared to 5-HT. Phospho-ERK1/2 levels are elevated following application of acetylcholine, but not significantly compared to baseline; n = 3 independent experiments carried out in triplicate; error bars represent SEM; *p <0.05; **p <0.01; ***p <0.001 (one way analysis of variance with Tukey post hoc analysis)