Fig. 3

hAEC transplantation reduced chemotherapy-induced inflammation in ovarian tissue. A Section of dissected hAECs illustrated a cobble stone-like epithelial cell morphology. B Relative expression of mRNA levels detected by real-time PCR in cultured hAECs. C Image of fixed ovarian tissue in different groups at 7 days after hAEC transplantation. D Bar graph demonstrated the ovarian weights in different groups. E H&E staining results displaying ovarian morphology in different groups at 7 days after hAEC transplantation. F Relative expression of inflammatory cytokine mRNA levels in injured ovarian tissue analyzed by real-time PCR at 7 days after hAEC transplantation. Data represent means ± SEM; *p < 0.05 versus Sham, #p < 0.05 versus Cy. Scale bar = 200 μm in (E). Cy chemoablated group (n = 6), Cy + hAECs hAEC-treated group (n = 6), Sham sham group (n = 4)