Fig. 6From: Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serumQuantitative RT-PCR in three-dimensional (3D) cell culture. The cells were cultured in 3D osteogenic conditions up to 21 days. Total RNA was isolated and the expression of Runx2 (a), alkaline phosphatase (ALP) (b), and osteocalcin (OCN) (c) was analyzed using quantitative RT-PCR. DIFF differentiation, FCS fetal calf serum, PLP platelet lysate and plasmaBack to article page