Fig. 7
Neurodegeneration enhances the neuroprotective features of rMAPC. a Neuroprotection assay where OLN93 cells were pre-treated (with the indicated (double) conditioned media and then three concentrations of H2O2 were added (pos. control). Optical densities are shown (±SEM) from five experiments. Negative control (dotted line) represents OLN93 cells without damage insult. One way analysis of variance followed by Dunnet’s test for multiple comparisons between groups was used. b OLN93 cells were pre-treated for 5 hours with rMAPC double conditioned media (DCM) and then allowed to proliferate for 24 hours. Optical densitites are shown (±SEM), n = 5 experiments. c rMAPC gene expression of neurotrophic factors (VEGFα, CNTF, GDNF) when treated with OLN-CMH2O2 and OLN-CM. Expressions were relatively quantified against the expression of YWHAZ and CycA. The fold differences are shown in comparison to OLN-CM (±SEM) from five experiments. Mann Whitney t-test was used for comparisons between two groups. Significant differences are shown with asterisks: *P ≤ 0.05, ** P ≤ 0.01, ***P ≤ 0.001. CM conditioned medium, CNTF ciliary neurotrophic factor, GDNF glial cell line-derived neurotrophic factor, OLN med OLN93 medium, VEGF vascular endothelial growth factor