Fig. 1From: Pre-differentiation of human neural stem cells into GABAergic neurons prior to transplant results in greater repopulation of the damaged brain and accelerates functional recovery after transient ischemic strokeUndifferentiated hNSCs and predifferentiated cell phenotypes in vitro. Immunofluorescent confocal images of undifferentiated SVZ-hNSCs in culture expressed HuNu (red) and SOX2 (green) (a), and Ki67 (red) and Nestin (green) (b), with colocalization giving a yellow appearance. Undifferentiated SVZ-hNSCs immunopositive for HuNu (red) (c–f) in culture were not observed to express Tuj1 (green) (c), GABA (green) (d), GAD (green) e, or DCX (green) f. Predifferentiated cells cultured for 7 days demonstrated immunoreactivity for HuNu (red) g–m and colabeled with Tuj1 (green) (g), GABA (green) (h), GAD (green) (i), and DCX (green) (j). Predifferentiated cells HuNu (red) cultured for 7 days demonstrated little immunoreactivity for Ki67 (green) (k), Nestin (green) (l), or SOX2 (green) (m). Scale bar: (a–f) 100 μm, (g–m) 50 μm. DCX doublecortin, GABA gamma-aminobutyric acid, GAD glutamate decarboxylase 65&67, HuNu human specific nuclear antigen, Tuj1 β-III tubulinBack to article page