Fig. 5
Evaluation of cell proliferation. a To determine the impact of FTH1 expression on cells, the proliferation of treated cells (5 × 103) cultured in a 96-well plate for 72 h was assessed using Cell Counting Kit-8 (CCK-8). A high dose of FAC (500 μM) inhibited the growth of both C3H10T1/2-WT cells in the presence or absence of Dox and C3H10T1/2-FTH1 cells only in the absence of Dox (P < 0.05). However, maximal FTH1 expression did not interfere with C3H10T1/2 cell proliferation in vitro, even in the presence of iron supplementation. b The impact of Dox on cell growth was also determined using CCK-8. There was no significant suppression of cell growth at a Dox concentration of 0.8 μg/ml. However, significant suppression of the growth of C3H10T1/2-FTH1 cells compared with C3H10T1/2-WT cells was detected at a Dox concentration of 2 μg/ml (P < 0.001, n = 5). At higher concentrations of Dox, the proliferation of both C3H10T1/2-WT and C3H10T1/2-FTH1 cells negatively correlated with the Dox concentration, although the C3H10T1/2-FTH1 cells showed a slightly steeper negative linear correlation than did the C3H10T1/2-WT cells. FTH1 ferritin heavy chain, FAC ferric ammonium citrate, Dox doxycycline