Fig 3From: Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditionsClinical-grade hiPSCs can randomly differentiate into all three germ layers and directly differentiate into functional neural cells and cardiomyocytes in Xeno-free media. a Morphology of EBs at day 8 derived from one clinical-grade hiPSC line. Scale bar, 200 μm. b Gene expression profile for germ-layer marker genes of the day 8 EBs, ectoderm (Gad1 and Pax6), mesoderm (Enolase and Osteonectin), endoderm (Nicastrin and Alpha-fetoprotein), and pluripotency (Oct4, Sox2 and Nanog). c Hematoxylin and eosin staining of teratoma derived from one clinical-grade hiPSC line. Scale bar, 100 μm. The teratomas contain tissues of all three germ layers. d Immunofluorescence results of neural cells differentiated from one clinical-grade hiPSC line. The neural cells express TH1 and TUJ1 at protein level. Scale bar, 100 μm. e Immunofluorescence results of cardiomyocytes differentiated from one clinical-grade hiPSC line. The cardiomyocytes express NKX2.5 and CTNT at protein level. Scale bar, 100 μm. EB embryoid body, GMP good manufacturing practice, iPSC induced pluripotent stem cellBack to article page