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Table 1 Primers for Sim1-Puro generation

From: A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons

Primer name

Sequence

pstartK_Sim1_upstream

AAAGTACTGTTTCTGGGGAAAACTCTAGTTTAGAGACCCTCCTGTTCTAAcgactgaattggttcctttaaagc

pstartK_Sim1_downstream

GAACCAGGCAGAGGGAAAGCTTCTCATTAGTGCTTTTCCCTTCTCTCTCCgccgcactcgagatatctagaccca

Sim1_CAT_Forward

GATGAGTCTGTGGAGTTTACGTTGTAAGAAGAAAGGGAGCCCGAGACACGGGCGCGCCagcattacacgtcttgagcgattgt

Sim1_CAT_Reverse 900 bp

TTGAGGAAGGGTGAGCAAATGGGAGATCAAAGAGCTCCTTCCCTGGAGAGGGCGCGCCcacttaacggctgacatgggaatta

Sim1_Fwd_Junction1

atgcacacgactcttcaaagaa

Puro_Reverse Junction1

gcgccaggaggccttccatctgttgct

  1. Capital letters indicate homology arms for red recombination
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Stem Cell Research & Therapy

ISSN: 1757-6512