Fig. 10

Mitosox Red Dye to detect ROS in MSCs in HG at Day-14 of culture, using FACS Analyzer: shows increased mitochondrial swelling, possibly secondary to ROS in high glucose state (red vs green). Blue- SCM (negative control), green- MSCs in normal glucose and red- MSCs in High Glucose, Orange: MSCs transduced with AdSOD2 and then exposed to high glucose. The figure on the right indicates that MSCs post SOD2 up-regulation (orange) have less fluorescence than without (red line). In both cases the MSCs were HG exposed. ROS reactive oxygen species, MSCs mesenchymal stem cells, FACS fluorescence activated cell sorting, SOD2 superoxide dismutase