Human adult stem cells derived from adipose tissue and bone marrow attenuate enteric neuropathy in the guinea-pig model of acute colitis

Table 3 Effects of mesenchymal stem cells derived from bone marrow and adipose tissue on nerve fibre density in TNBS-induced colitis

	Sham	TNBS	TNBS + BM-MSC	TNBS + AT-MSC
	Level of CGRP immunoreactivity/1 mm² (%)
24 h	12.0 ± 0.1	6.3 ± 0.6 ††††	9.5 ± 0.5 *** ††	8.5 ± 0.4 * †††
72 h	12.2 ± 0.4	6.1 ± 0.4 ††††	9.6 ± 0.7 *** ††	9.4 ± 0.3 *** ††
	Density of TH-IR nerve fibres/1 mm² (%)
24 h	17.2 ± 0.8	11.6 ± 0.4 ††	17.4 ± 0.1 **	16.8 ± 0.9 **
72 h	17.4 ± 1.1	12.6 ± 0.4 ††	17.0 ± 1.3 *	17.5 ± 1.2 **
	Density of VAChT-IR nerve fibres/1 mm² (%)
24 h	19.9 ± 0.9	11.6 ± 0.8 ††††	20.3 ± 0.5 ****	19.9 ± 0.2 ****
72 h	20.5 ± 1.1	12.9 ± 0.4 ††††	19.6 ± 0.7 ****	20.0 ± 0.6 ****

TNBS 2,4,6-trinitrobenzene sulfonic acid, BM-MSC bone marrow derived mesenchymal stem cells, AT-MSC adipose tissue derived mesenchymal stem cells, CGRP calcitonin gene-related peptide, TH tyrosine hydroxylase, VAChT vesicular acetylcholine transporter, IR immunoreactive
*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, significantly different to TNBS; ††p < 0.01, †††p < 0.001, ††††p < 0.0001, significantly different to sham

ISSN: 1757-6512